Back to Search Start Over

Validation of a Novel Three-Dimensional (3D Fusion) Gross Sampling Protocol for Clear Cell Renal Cell Carcinoma to Overcome Intratumoral Heterogeneity: The Meet-Uro 18 Study

Authors :
Matteo Brunelli
Guido Martignoni
Giorgio Malpeli
Alessandro Volpe
Luca Cima
Maria Rosaria Raspollini
Mattia Barbareschi
Alessandro Tafuri
Giulia Masi
Luisa Barzon
Serena Ammendola
Manuela Villanova
Maria Angela Cerruto
Michele Milella
Sebastiano Buti
Melissa Bersanelli
Giuseppe Fornarini
Sara Elena Rebuzzi
Valerio Gaetano Vellone
Gabriele Gaggero
Giuseppe Procopio
Elena Verzoni
Sergio Bracarda
Martina Fanelli
Roberto Sabbatini
Rodolfo Passalacqua
Bruno Perrucci
Maria Olga Giganti
Maddalena Donini
Stefano Panni
Marcello Tucci
Veronica Prati
Cinzia Ortega
Anna Caliò
Albino Eccher
Filippo Alongi
Giovanni Pappagallo
Roberto Iacovelli
Alessandra Mosca
Paolo Umari
Ilaria Montagnani
Stefano Gobbo
Francesco Atzori
Enrico Munari
Marco Maruzzo
Umberto Basso
Francesco Pierconti
Carlo Patriarca
Piergiuseppe Colombo
Alberto Lapini
Giario Conti
Roberto Salvioni
Enrico Bollito
Andrea Cossarizza
Francesco Massari
Mimma Rizzo
Renato Franco
Federica Zito-Marino
Yoseba Aberasturi Plata
Francesca Galuppini
Marta Sbaraglia
Matteo Fassan
Angelo Paolo Dei Tos
Maurizio Colecchia
Holger Moch
Maurizio Scaltriti
Camillo Porta
Brett Delahunt
Gianluca Giannarini
Roberto Bortolus
Pasquale Rescigno
Giuseppe Luigi Banna
Alessio Signori
Miguel Angel Llaja Obispo
Roberto Perris
Alessandro Antonelli
Brunelli, Matteo
Martignoni, Guido
Malpeli, Giorgio
Volpe, Alessandro
Cima, Luca
Raspollini, Maria Rosaria
Barbareschi, Mattia
Tafuri, Alessandro
Masi, Giulia
Barzon, Luisa
Ammendola, Serena
Villanova, Manuela
Cerruto, Maria Angela
Milella, Michele
Buti, Sebastiano
Bersanelli, Melissa
Fornarini, Giuseppe
Rebuzzi, Sara Elena
Vellone, Valerio Gaetano
Gaggero, Gabriele
Procopio, Giuseppe
Verzoni, Elena
Bracarda, Sergio
Fanelli, Martina
Sabbatini, Roberto
Passalacqua, Rodolfo
Perrucci, Bruno
Giganti, Maria Olga
Donini, Maddalena
Panni, Stefano
Tucci, Marcello
Prati, Veronica
Ortega, Cinzia
Caliò, Anna
Eccher, Albino
Alongi, Filippo
Pappagallo, Giovanni
Iacovelli, Roberto
Mosca, Alessandra
Umari, Paolo
Montagnani, Ilaria
Gobbo, Stefano
Atzori, Francesco
Munari, Enrico
Maruzzo, Marco
Basso, Umberto
Pierconti, Francesco
Patriarca, Carlo
Colombo, Piergiuseppe
Lapini, Alberto
Conti, Giario
Salvioni, Roberto
Bollito, Enrico
Cossarizza, Andrea
Massari, Francesco
Rizzo, Mimma
Franco, Renato
Zito-Marino, Federica
Aberasturi Plata, Yoseba
Galuppini, Francesca
Sbaraglia, Marta
Fassan, Matteo
Dei Tos, Angelo Paolo
Colecchia, Maurizio
Moch, Holger
Scaltriti, Maurizio
Porta, Camillo
Delahunt, Brett
Giannarini, Gianluca
Bortolus, Roberto
Rescigno, Pasquale
Banna, Giuseppe Luigi
Signori, Alessio
Obispo, Miguel Angel Llaja
Perris, Roberto
Antonelli, Alessandro
Brunelli M.
Martignoni G.
Malpeli G.
Volpe A.
Cima L.
Raspollini M.R.
Barbareschi M.
Tafuri A.
Masi G.
Barzon L.
Ammendola S.
Villanova M.
Cerruto M.A.
Milella M.
Buti S.
Bersanelli M.
Fornarini G.
Rebuzzi S.E.
Vellone V.G.
Gaggero G.
Procopio G.
Verzoni E.
Bracarda S.
Fanelli M.
Sabbatini R.
Passalacqua R.
Perrucci B.
Giganti M.O.
Donini M.
Panni S.
Tucci M.
Prati V.
Ortega C.
Calio A.
Eccher A.
Alongi F.
Pappagallo G.
Iacovelli R.
Mosca A.
Umari P.
Montagnani I.
Gobbo S.
Atzori F.
Munari E.
Maruzzo M.
Basso U.
Pierconti F.
Patriarca C.
Colombo P.
Lapini A.
Conti G.
Salvioni R.
Bollito E.
Cossarizza A.
Massari F.
Rizzo M.
Franco R.
Zito-Marino F.
Plata Y.A.
Galuppini F.
Sbaraglia M.
Fassan M.
Dei Tos A.P.
Colecchia M.
Moch H.
Scaltriti M.
Porta C.
Delahunt B.
Giannarini G.
Bortolus R.
Rescigno P.
Banna G.L.
Signori A.
Obispo M.A.L.
Perris R.
Antonelli A.
Source :
Journal of Personalized Medicine; Volume 12; Issue 5; Pages: 727
Publication Year :
2022

Abstract

We aimed to overcome intratumoral heterogeneity in clear cell renal cell carcinoma (clearRCC). One hundred cases of clearRCC were sampled. First, usual standard sampling was applied (1 block/cm of tumor); second, the whole tumor was sampled, and 0.6 mm cores were taken from each block to construct a tissue microarray; third, the residual tissue, mapped by taking pieces 0.5 × 0.5 cm, reconstructed the entire tumor mass. Precisely, six randomly derived pieces of tissues were placed in each cassette, with the number of cassettes being based on the diameter of the tumor (called multisite 3D fusion). Angiogenic and immune markers were tested. Routine 5231 tissue blocks were obtained. Multisite 3D fusion sections showed pattern A, homogeneous high vascular density (10%), pattern B, homogeneous low vascular density (8%) and pattern C, heterogeneous angiogenic signatures (82%). PD-L1 expression was seen as diffuse (7%), low (33%) and absent (60%). Tumor-infiltrating CD8 scored high in 25% (pattern hot), low in 65% (pattern weak) and zero in 10% of cases (pattern desert). Grading was upgraded in 26% of cases (G3–G4), necrosis and sarcomatoid/rhabdoid characters were observed in, respectively, 11 and 7% of cases after 3D fusion (p = 0.03). CD8 and PD-L1 immune expressions were higher in the undifferentiated G4/rhabdoid/sarcomatoid clearRCC subtypes (p = 0.03). Again, 22% of cases were set to intermediate to high risk of clinical recurrence due to new morphological findings of all aggressive G4, sarcomatoid/rhabdoid features by using 3D fusion compared to standard methods (p = 0.04). In conclusion, we propose an easy-to-apply multisite 3D fusion sampling that negates bias due to tumor heterogeneity.

Details

Language :
English
Database :
OpenAIRE
Journal :
Journal of Personalized Medicine; Volume 12; Issue 5; Pages: 727
Accession number :
edsair.doi.dedup.....e71a28bdaec6bee7f85bf562d57a3b6b