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Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2−/− mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Pathology
medicine.medical_specialty
Neutrophils
Lipoproteins
Interleukin-1beta
Primary Cell Culture
Inflammation
Biology
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Downregulation and upregulation
medicine
Cell Adhesion
Animals
Humans
Platelet Activating Factor
Lung
Peroxidase
Mice, Knockout
Multidisciplinary
Platelet-activating factor
Tumor Necrosis Factor-alpha
Escherichia coli Proteins
Survival Analysis
Endotoxemia
Mice, Inbred C57BL
TLR2
030104 developmental biology
RAW 264.7 Cells
chemistry
Gene Expression Regulation
Liver
Cell culture
Cyclooxygenase 2
030220 oncology & carcinogenesis
Macrophages, Peritoneal
lipids (amino acids, peptides, and proteins)
medicine.symptom
Bacterial outer membrane
Ex vivo
Polymyxin B
medicine.drug
Bacterial Outer Membrane Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....e712bafcc27a824193894b97b7768bff