Back to Search
Start Over
Simulated biological fluid exposure changes nanoceria’s surface properties but not its biological response
- Source :
- Eur J Pharm Biopharm
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Nanoscale cerium dioxide (nanoceria) has industrial applications, capitalizing on its catalytic, abrasive, and energy storage properties. It auto-catalytically cycles between Ce(3+) and Ce(4+), giving it pro-and anti-oxidative properties. The latter mediates beneficial effects in models of diseases that have oxidative stress/inflammation components. Engineered nanoparticles become coated after body fluid exposure, creating a corona, which can greatly influence their fate and effects. Very little has been reported about nanoceria surface changes and biological effects after pulmonary or gastrointestinal fluid exposure. The study objective was to address the hypothesis that simulated biological fluid (SBF) exposure changes nanoceria’s surface properties and biological activity. This was investigated by measuring the physicochemical properties of nanoceria with a citric acid coating (size; morphology; crystal structure; surface elemental composition, charge, and functional groups; and weight) before and after exposure to simulated lung, gastric, and intestinal fluids. SBF-exposed nanoceria biological effect was assessed as A549 or Caco-2 cell resazurin metabolism and mitochondrial oxygen consumption rate. SBF exposure resulted in loss or overcoating of nanoceria’s surface citrate, greater nanoceria agglomeration, deposition of some SBF components on nanoceria’s surface, and small changes in its zeta potential. The engineered nanoceria and SBF-exposed nanoceria produced no statistically significant changes in cell viability or cellular oxygen consumption rates.
- Subjects :
- Cell Survival
Surface Properties
Cellular respiration
Pharmaceutical Science
chemistry.chemical_element
02 engineering and technology
engineering.material
medicine.disease_cause
030226 pharmacology & pharmacy
Oxygen
Article
03 medical and health sciences
chemistry.chemical_compound
Oxygen Consumption
0302 clinical medicine
Coating
Cell Line, Tumor
medicine
Zeta potential
Humans
Biological activity
Resazurin
Cerium
General Medicine
021001 nanoscience & nanotechnology
Body Fluids
Mitochondria
Oxidative Stress
chemistry
A549 Cells
engineering
Biophysics
Nanoparticles
Caco-2 Cells
0210 nano-technology
Citric acid
Oxidation-Reduction
Oxidative stress
Biotechnology
Subjects
Details
- ISSN :
- 09396411
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmaceutics and Biopharmaceutics
- Accession number :
- edsair.doi.dedup.....e6e8d41fa2bdaaeccf084954c104bbab