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A hyperactive transcriptional state marks genome reactivation at the mitosis–G1 transition
- Publication Year :
- 2016
- Publisher :
- Cold Spring Harbor Laboratory Press, 2016.
-
Abstract
- During mitosis, RNA polymerase II (Pol II) and many transcription factors dissociate from chromatin, and transcription ceases globally. Transcription is known to restart in bulk by telophase, but whether de novo transcription at the mitosis–G1 transition is in any way distinct from later in interphase remains unknown. We tracked Pol II occupancy genome-wide in mammalian cells progressing from mitosis through late G1. Unexpectedly, during the earliest rounds of transcription at the mitosis–G1 transition, ∼50% of active genes and distal enhancers exhibit a spike in transcription, exceeding levels observed later in G1 phase. Enhancer–promoter chromatin contacts are depleted during mitosis and restored rapidly upon G1 entry but do not spike. Of the chromatin-associated features examined, histone H3 Lys27 acetylation levels at individual loci in mitosis best predict the mitosis–G1 transcriptional spike. Single-molecule RNA imaging supports that the mitosis–G1 transcriptional spike can constitute the maximum transcriptional activity per DNA copy throughout the cell division cycle. The transcriptional spike occurs heterogeneously and propagates to cell-to-cell differences in mature mRNA expression. Our results raise the possibility that passage through the mitosis–G1 transition might predispose cells to diverge in gene expression states.
- Subjects :
- 0301 basic medicine
Transcriptional Activation
Erythroblasts
Mitosis
RNA polymerase II
Enhancer RNAs
Cell Line
03 medical and health sciences
Mice
Transcription (biology)
Genetics
Animals
Enhancer
Promoter Regions, Genetic
Transcription factor
Cells, Cultured
Genome
biology
Cell Cycle
G1 Phase
Molecular biology
Chromatin
Up-Regulation
030104 developmental biology
Terminator (genetics)
Enhancer Elements, Genetic
biology.protein
DNA, Intergenic
Developmental Biology
Research Paper
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e6e1e0e4c7ed48bc3709b4a3017d8a29