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Lipo‐chitooligosaccharides promote lateral root formation and modify auxin homeostasis in Brachypodium distachyon
- Source :
- New Phytologist, New Phytologist, Wiley, 2018, 221 (4), pp.2190-2202. ⟨10.1111/nph.15551⟩, New Phytologist, Wiley, 2019, 221 (4), pp.2190-2202. ⟨10.1111/nph.15551⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; . Lipo-chitooligosaccharides (LCOs) are microbial symbiotic signals that also influence root growth. In Medicago truncatula, LCOs stimulate lateral root formation (LRF) synergistically with auxin. However, the molecular mechanisms of this phenomenon and whether it is restricted to legume plants are not known. . We have addressed the capacity of the model monocot Brachypodium distachyon (Brachypodium) to respond to LCOs and auxin for LRF. For this, we used a combination of root phenotyping assays, live-imaging and auxin quantification, and analysed the regulation of auxin homeostasis genes. We show that LCOs and a low dose of the auxin precursor indole-3-butyric acid (IBA) stimulated LRF in Brachypodium, while a combination of LCOs and IBA led to different regulations. Both LCO and IBA treatments locally increased endogenous indole-3-acetic acid (IAA) content, whereas the combination of LCO and IBA locally increased the endogenous concentration of a conjugated form of IAA (IAA-Ala). LCOs, IBA and the combination differentially controlled expression of auxin homeostasis genes. . These results demonstrate that LCOs are active on Brachypodium roots and stimulate LRF probably through regulation of auxin homeostasis. The interaction between LCO and auxin treatments observed in Brachypodium on root architecture opens interesting avenues regarding their possible combined effects during the arbuscular mycorrhizal symbiosis.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Indoles
hormone homeostasis
Physiology
Oligosaccharides
lateral root primordia
Chitin
Endogeny
Plant Science
root architecture
indole-3-butyric acid (IBA)
Models, Biological
Plant Roots
01 natural sciences
Fluorescence
03 medical and health sciences
lipochitooligosaccharide (LCO)
Auxin
Nod factors
Homeostasis
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
heterocyclic compounds
Gene
Lateral root formation
ComputingMilieux_MISCELLANEOUS
chemistry.chemical_classification
Chitosan
Indoleacetic Acids
biology
Auxin homeostasis
Chemistry
fungi
food and beverages
biology.organism_classification
Lipids
Medicago truncatula
Cell biology
auxin dosage
030104 developmental biology
Brachypodium
Brachypodium distachyon
symbiotic signal
Signal Transduction
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 0028646X and 14698137
- Database :
- OpenAIRE
- Journal :
- New Phytologist, New Phytologist, Wiley, 2018, 221 (4), pp.2190-2202. ⟨10.1111/nph.15551⟩, New Phytologist, Wiley, 2019, 221 (4), pp.2190-2202. ⟨10.1111/nph.15551⟩
- Accession number :
- edsair.doi.dedup.....e6da799093a61a18a292fff48ad2bbd2