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Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase
- Source :
- FEBS letters. 488(1-2)
- Publication Year :
- 2001
-
Abstract
- In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.
- Subjects :
- Cell Extracts
Biophysics
Brassica
Biology
Reductase
Tritium
Biochemistry
Fatty acid synthase
chemistry.chemical_compound
Biosynthesis
Structural Biology
Genetics
Molecular Biology
Fatty acid synthesis
Brassica napus
Fatty Acids
Cell Biology
biology.organism_classification
[3H]NADPH
Enzyme assay
Plant Leaves
Specific antibody
Alcohol Oxidoreductases
Kinetics
chemistry
Spectrophotometry
3-Oxoacyl-(Acyl-Carrier-Protein) Reductase
biology.protein
3-Oxoacyl-ACP reductase
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 488
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....e6cc56606882f4c00b880b8a4d822675