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Photo-affinity labelling at the peptidyl transferase centre reveals two different positions for the A- and P-sites in domain V of 23S rRNA
- Source :
- Europe PubMed Central, Scopus-Elsevier
- Publication Year :
- 1988
- Publisher :
- Wiley, 1988.
-
Abstract
- Photo-reactive 3-(4'-benzoylphenyl)propionyl-Phe-tRNA bound to the A- or the P-site was crosslinked to 23S RNA upon irradiation at 320 nm. The sites of reaction were identified as U-2584 and U-2585 at the A-site and A-2451 and C-2452 at the P-site. Minor crosslinks from both sites were observed at nucleotides A-2503 to U-2506. All sites identified lie in close proximity according to the secondary structure model and constitute part of the highly conserved loop region of domain V. Antibiotics known to inhibit peptidyl transferase activity had a pronounced effect on photo-crosslinking. In addition, tetracycline was also shown to photo-crosslink to this region. These experiments permit a dissection of the peptidyl transferase region on the 23S RNA into two distinct areas for the A- and P-site.
- Subjects :
- Peptidyl transferase
Tetracycline
macromolecular substances
RNA, Transfer, Amino Acyl
General Biochemistry, Genetics and Molecular Biology
RNA, Transfer, Phe
23S ribosomal RNA
Labelling
Escherichia coli
medicine
Nucleotide
Molecular Biology
Protein secondary structure
chemistry.chemical_classification
Binding Sites
General Immunology and Microbiology
biology
General Neuroscience
technology, industry, and agriculture
RNA
Affinity Labels
RNA, Transfer, Amino Acid-Specific
Anti-Bacterial Agents
RNA, Ribosomal, 23S
Biochemistry
chemistry
RNA, Ribosomal
Protein Biosynthesis
Peptidyl Transferases
Domain (ring theory)
biology.protein
Nucleic Acid Conformation
Ribosomes
Acyltransferases
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 02614189
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....e6c4bbeda609ab10be73268f27faa7d6