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Establishment and characterization of a cytotrophoblast cell line from normal placenta of human origin
- Source :
- Human Reproduction. 11:1328-1333
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- A cell line has been established from human placentae at the first trimester of normal pregnancy. The cell line was obtained by culture of purified cytotrophoblast cells in serum-free medium supplemented with epidermal growth factor, insulin, dexamethasone and 0.1% bovine serum albumin. The cells can be subcultured for >30 passages in one to three splits. All the cells were mononuclear epithelial-like cells positive to cytokeratin 18, gonadotrophin-releasing hormone (GnRH), neuropeptide Y, neurotensin, leucine-enkephalin, dopamine and 5-hydroxytryptamine immunocytochemical staining. The cells secreted GnRH, progesterone and oestradiol (in the presence of testosterone) but little human chorionic gonadotrophin and no beta-endorphin. The cell line showed human karyotypes and had a population doubling time of 48 h in serum-free medium. However, the cells would stop growing in the medium containing fetal bovine serum. A normal cytotrophoblast cell line established in serum-free medium will be particularly useful in the study of cytotrophoblast cell proliferation and differentiation.
- Subjects :
- endocrine system
medicine.medical_specialty
Cell division
medicine.drug_class
Placenta
Mice, Nude
Biology
Chorionic Gonadotropin
Cell Line
Gonadotropin-Releasing Hormone
Andrology
Mice
Pregnancy
Epidermal growth factor
Internal medicine
Cell Adhesion
medicine
Animals
Humans
Progesterone
reproductive and urinary physiology
Cytotrophoblast
Cell growth
Rehabilitation
Chromosome Mapping
Obstetrics and Gynecology
Trophoblasts
Pregnancy Trimester, First
medicine.anatomical_structure
Endocrinology
Reproductive Medicine
Cell culture
embryonic structures
Female
Gonadotropin
Cell Division
hormones, hormone substitutes, and hormone antagonists
Fetal bovine serum
Subjects
Details
- ISSN :
- 14602350 and 02681161
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Human Reproduction
- Accession number :
- edsair.doi.dedup.....e6ab0cd5f167f73ad6bf7194868c6d67