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Crucial role of leaky initiation of uORF3 in the downregulation of HNT1 by ER stress
- Source :
- Biochemical and Biophysical Research Communications. 528:186-192
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- eIF2α phosphorylation-mediated translational regulation is crucial for global repression of translation by various stresses, including the unfolded protein response (UPR) in eukaryotes. Although translational control during UPR has not been extensively investigated in S. cerevisiae, Hac1-mediated production of long transcripts containing uORFs was shown to repress the translation of histidine triad nucleotide-binding 1 (HNT1) mRNA. The present study showed that uORF3 is required for HNT1 expression, as well as down-regulating HNT1 translation. Translation initiation by uORF3 is inefficient, with uORF3 having a strong Kozak sequence efficiently repressing the translation of HNT1. We propose that leaky scanning of uORF3 is responsible for the downregulation of HNT1 during UPR.
- Subjects :
- 0301 basic medicine
Saccharomyces cerevisiae Proteins
Hydrolases
Kozak consensus sequence
Biophysics
Down-Regulation
Leaky scanning
Saccharomyces cerevisiae
Biochemistry
03 medical and health sciences
0302 clinical medicine
Eukaryotic translation
Downregulation and upregulation
Gene Expression Regulation, Fungal
Translational regulation
Molecular Biology
Psychological repression
Base Sequence
Chemistry
Translation (biology)
Cell Biology
Endoplasmic Reticulum Stress
Cell biology
030104 developmental biology
030220 oncology & carcinogenesis
Unfolded Protein Response
Unfolded protein response
5' Untranslated Regions
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 528
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....e6877615e1940818d52598ab32bf921e