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Characterization of folate production and probiotic potential of Streptococcus gallolyticus subsp. macedonicus CRL415
- Source :
- Food Microbiology. 79:20-26
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Mandatory fortification of foods with folic acid is being questioned by many scientists principally because of the potential adverse secondary effects associated with their excessive consumption. It has been shown that selected strains of lactic acid bacteria (LAB) are able to produce natural forms of folate and these could be included in foods to prevent deficiencies without causing adverse effects. The aim of this study was to evaluate folate production and fol gene expression by Streptococcus gallolyticus subsp. macedonicus (S. macedonicus) CRL415 under different growth conditions in vitro and to assess its potential probiotic application. Results showed that glucose as the principal carbon source, and incubation at 42 °C under controlled pH conditions (6.0) increased folate production, fol gene expression, and growth of S. macedonicus CRL415. This strain was able to produce elevated folate concentrations during milk fermentation without the need of prolonged incubation times and was able to resist conditions simulating the gastrointestinal tract. In addition, S. macedonicus was susceptible to all required antibiotics, and had a good adhesion level to intestinal cells in vitro, making it a promising candidate for biotechnological application as functional starter cultures in the dairy industry. Fil: Laiño, Jonathan Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Levit, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Savoy, Graciela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
- Subjects :
- Cultured Milk Products
medicine.drug_class
Antibiotics
Microbial Sensitivity Tests
FOL GENES EXPRESSION
Microbiology
Bacterial Adhesion
law.invention
Ciencias Biológicas
03 medical and health sciences
chemistry.chemical_compound
Probiotic
Folic Acid
Starter
Biología Celular, Microbiología
LACTIC ACID BACTERIA
law
FOLATE PRODUCTION
medicine
Animals
Bile
Humans
Streptococcus gallolyticus
Food science
Incubation
STREPTOCOCCUS GALLOLYTICUS SUBSP. MACEDONICUS
030304 developmental biology
0303 health sciences
Gastric Juice
Microbial Viability
biology
030306 microbiology
Probiotics
PROBIOTIC PROPERTIES
Temperature
Streptococcus
Gene Expression Regulation, Bacterial
Hydrogen-Ion Concentration
biology.organism_classification
Anti-Bacterial Agents
Lactic acid
Glucose
chemistry
Fermentation
Caco-2 Cells
4-Aminobenzoic Acid
CIENCIAS NATURALES Y EXACTAS
Bacteria
Food Science
Subjects
Details
- ISSN :
- 07400020
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Food Microbiology
- Accession number :
- edsair.doi.dedup.....e67e10d323c5208eccfef22db670b63f