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An advanced in vitro model to assess glaucoma onset
- Publication Year :
- 2020
- Publisher :
- Springer Spektrum, 2020.
-
Abstract
- Glaucoma is the second leading cause of blindness worldwide. Currently, glaucoma treatments aim to lower intraocular pressure by decreasing aqueous humor production or increasing aqueous humor outflow through pharmacological approaches or trabeculectomy. The lack of an effective cure requires new therapeutic strategies. We compared the bio­logical responses of a three-dimensional trabecular meshwork model with or without perfusion bioreactor technology to better understand the early molecular changes induced by prolonged oxidative stress conditions induced by repeated daily peroxide exposure. We used standard 3D cultures of trabecular meshwork cells in Matrigel cultured under either static and dynamic conditions for one week. We studied changes in F-actin expression and organization in the cells, cellular metabolic activity, proinflammatory gene expression, expression of pro- and anti-apoptotic proteins, PARP-1 cleavage, and NFκB activation in the model. We demonstrate that the dynamic conditions improve the adaptive behavior of 3D trabecular meshwork cultures to chronic oxidative stress via offsetting pathway activation.
- Subjects :
- 0301 basic medicine
Intraocular pressure
genetic structures
medicine.medical_treatment
Cell Culture Techniques
Glaucoma
Pharmacology
medicine.disease_cause
Animal Testing Alternatives
trabecular meshwork cells
Proinflammatory cytokine
03 medical and health sciences
bioreactor
0302 clinical medicine
Trabecular Meshwork
Medicine
Trabeculectomy
Humans
Chronic stress
Matrigel
3D cultures
business.industry
General Medicine
medicine.disease
eye diseases
Actins
Medical Laboratory Technology
Drug Combinations
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
in vitro model
Proteoglycans
sense organs
Trabecular meshwork
Collagen
Laminin
business
3D cultures, bioreactor, in vitro model, trabecular meshwork cells
030217 neurology & neurosurgery
Oxidative stress
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....e6760bc2ae1706b5555ddb3ec2119df7