E-2 Glycoprotein structural variations analyzed within the CSFV 2.2. genogroup in a 'Closed Grid' sampling study from Meghalaya, India

Representative positive samples from the Khasi Hills region were selected for sequencing and “close grid” phylogenetic relationship using partial genomic regions of 5’UTR and E2. High seroprevalence (74.7%) of CSFV was recorded. Detection of the CSFV genome in serological positive serum samples and tissue samples was 61.29% and 18.94%, respectively. BLAST and phylogenetic analysis indicate the clustering of all the field samples in subgroup 2.2 with high identity with EF014334 from China. Molecular structural modeling of the E2 partial sequence using representative sequences MG563797 from Meghalaya and EF014334 from China indicate potential changes in the protein motif and its conformation that may indicate the reason for the emergence of subgroup 2.2 CSFV replacing the predominant subgroup 1.1 viruses in North-East India.&nbsp