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The hymenochirins: A family of host-defense peptides from the Congo dwarf clawed frog Hymenochirus boettgeri (Pipidae)
- Source :
- Peptides, Peptides, Elsevier, 2012, 35 (2), pp.269-275. ⟨10.1016/j.peptides.2012.03.029⟩
- Publication Year :
- 2012
- Publisher :
- HAL CCSD, 2012.
-
Abstract
- International audience; Skin secretions of frogs from the subfamily Xenopodinae (Xenopus+Silurana) within the family Pipidae are a rich source of antimicrobial peptides with therapeutic potential but species from the sister taxon Hymenochirus in the subfamily Pipinae (Hymenochirus+Pseudhymenochirus+Pipa) have not been investigated. Peptidomic analysis of norepinephrine-stimulated skin secretions from two distinct populations of the Congo dwarf clawed frog Hymenochirus boettgeri (Tornier, 1896) has led to identification of five structurally related peptides with broad-spectrum antimicrobial activity. Hymenochirin-1B (IKLSPETKDNLKKVLKGAIKGAIAVAKMV.NH(2)) is C-terminally α-amidated whereas hymenochirins-2B-5B have the general structure XKIPX(2)VKDTLKKVAKGX(2)SX(2)AGAX(3).COOH. Hymenochirin-3B (IKIPAVVKDTLKKVAKGVLSAVAGALTQ) was the most abundant peptide in the secretions. The hymenochirins show very low structural similarity with the antimicrobial peptides isolated from skin secretions of Silurana tropicalis and Xenopus laevis consistent with the proposed ancient divergence of the Pipinae and Xenopodinae. Synthetic replicates of hymenochirin-1B-4B inhibit the growth of Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus (MIC in the range 10-40 μM) and Candida albicans (MIC=80 μM). The peptides display relatively weak hemolytic activity against human erythrocytes (LC(50) in the range 160 to >300 μM).
- Subjects :
- Pipidae
Subfamily
Erythrocytes
Physiology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Klebsiella pneumoniae
Xenopus
MESH: Amino Acid Sequence
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
medicine.disease_cause
Biochemistry
0302 clinical medicine
Endocrinology
Anti-Infective Agents
Candida albicans
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Staphylococcus aureus
MESH: Animals
Silurana
Skin
0303 health sciences
MESH: Microbial Sensitivity Tests
Hymenochirus boettgeri
biology
MESH: Escherichia coli
MESH: Erythrocytes
MESH: Antimicrobial Cationic Peptides
3. Good health
Klebsiella pneumoniae
Staphylococcus aureus
Pseudomonas aeruginosa
MESH: Pseudomonas aeruginosa
Antimicrobial peptides
MESH: Anti-Infective Agents
MESH: Sequence Alignment
Microbial Sensitivity Tests
Microbiology
03 medical and health sciences
Cellular and Molecular Neuroscience
MESH: Skin
Botany
medicine
Escherichia coli
Animals
Amino Acid Sequence
030304 developmental biology
MESH: Pipidae
MESH: Candida albicans
biology.organism_classification
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Frog Skin
Sequence Alignment
030217 neurology & neurosurgery
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 01969781
- Database :
- OpenAIRE
- Journal :
- Peptides, Peptides, Elsevier, 2012, 35 (2), pp.269-275. ⟨10.1016/j.peptides.2012.03.029⟩
- Accession number :
- edsair.doi.dedup.....e6676ad23c7827361356ba8533813ac9