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Experimental infection of cats with <scp> Cystoisospora felis </scp>
- Source :
- Journal of Veterinary Internal Medicine
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Background Cystoisospora felis is a common parasite of cats and is diagnosed by fecal flotation, but false-negative results can be common. Hypothesis/objectives To experimentally inoculate cats with C. felis oocysts, to compare fecal flotation and polymerase chain reaction (PCR) results, and to describe any clinical signs consistent with infection. Animals Six cats. Methods Cystoisospora felis oocysts were identified morphologically from feces of a naturally infected kitten with diarrhea, sporulated oocysts (5000) were inoculated to 6 cats that were negative for fecal parasites by fecal flotation and by a fluorescent antibody assay (FA) for Giardia spp. and Cryptosporidium spp. Cats were observed daily for the presence of clinical signs consistent with infection. Fecal samples were evaluated by fecal flotation and FA up to 3 times per week post inoculation (PI) to Day 27. Thirty-six samples collected before inoculation and from Days 8, 10, 13, 15, and 20 PI were assayed using an internal transcribed spacer 1 (ITS1) PCR that amplifies DNA of C. felis. Results All cats were negative for C. felis by both assays before inoculation. All cats shed C. felis oocysts by Day 10 PI, oocysts were not detected by fecal flotation after Day 15 PI. Cystoisospora felis DNA was amplified from 24/36 (66.6%) fecal samples from 6/6 (100%) of the cats. Oocysts were not detected by fecal flotation in 4 of the samples that were positive for C. felis DNA by PCR. Clinical signs were not recognized in any of the study cats. Conclusions and clinical importance Fecal flotation is a convenient assay for detection of C. felis but could occasionally give false-negative results when compared to this ITS1 PCR.
- Subjects :
- 040301 veterinary sciences
Cystoisospora felis
fecal flotation
Cryptosporidiosis
Cryptosporidium
Infectious Disease
Standard Article
030204 cardiovascular system & hematology
Cat Diseases
Microbiology
law.invention
0403 veterinary science
Feces
03 medical and health sciences
0302 clinical medicine
law
Felis
medicine
Animals
Parasite hosting
Parasites
Polymerase chain reaction
CATS
General Veterinary
biology
business.industry
04 agricultural and veterinary sciences
biology.organism_classification
Standard Articles
Diarrhea
Cats
Female
SMALL ANIMAL
clinical signs
medicine.symptom
GIARDIA SPP
business
ITS‐1 PCR
Subjects
Details
- ISSN :
- 19391676 and 08916640
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Internal Medicine
- Accession number :
- edsair.doi.dedup.....e619fda4d7ede9cab1af9c563641f476