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Characterization of the Small Intestinal Lesion in Celiac Disease by Label-Free Quantitative Mass Spectrometry
- Source :
- The American journal of pathology. 188(7)
- Publication Year :
- 2017
-
Abstract
- Global characterization of tissue proteomes from small amounts of biopsy material has become feasible due to advances in mass spectrometry and bioinformatics tools. In celiac disease (CD), dietary gluten induces an immune response which is accompanied by pronounced remodeling of the small intestine. Removal of gluten from the diet abrogates the immune response and the tissue architecture normalizes. In this study differences in global protein expression of small intestinal biopsies from CD patients were quantified by analyzing formalin-fixed, paraffin-embedded material using liquid-chromatography mass spectrometry and label-free protein quantitation. Protein expression was compared in biopsies collected from the same patients before and after one year treatment with gluten-free diet (n=10) or before and after three-day gluten provocation (n=4). Differential expression of proteins in particular from mature enterocytes, neutrophils, and plasma cells could distinguish untreated from treated CD mucosa, and immunoglobulin variable region IGHV5-51 expression was found to serve as a CD-specific marker of ongoing immune activation. In patients that had undergone gluten challenge coordinated up-regulation of wound response proteins including the CD autoantigen transglutaminase 2 was observed. Our study provides a global and unbiased assessment of antigen-driven changes in protein expression in the celiac intestinal mucosa.
- Subjects :
- 0301 basic medicine
Adult
Male
Pathology
medicine.medical_specialty
Proteome
Tissue transglutaminase
Quantitative proteomics
Mass Spectrometry
Pathology and Forensic Medicine
03 medical and health sciences
Diet, Gluten-Free
Young Adult
Immune system
Intestinal mucosa
Intestine, Small
medicine
Humans
chemistry.chemical_classification
biology
Middle Aged
Gluten
Small intestine
Celiac Disease
Intestinal Diseases
030104 developmental biology
medicine.anatomical_structure
chemistry
biology.protein
Gluten free
Female
Biomarkers
Subjects
Details
- ISSN :
- 15252191 and 00029440
- Volume :
- 188
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The American journal of pathology
- Accession number :
- edsair.doi.dedup.....e61971428c4fabbef259e370640355a0