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Subnanogram-concentration measurement of buprenorphine in human plasma by electron-capture capillary gas chromatography: application to pharmacokinetics of sublingual buprenorphine
- Source :
- Clinical chemistry. 43(12)
- Publication Year :
- 1998
-
Abstract
- We describe a sensitive and specific method for the measurement of buprenorphine in human plasma. The method involves a structural analog as an internal calibrator, careful control of pH during sample extraction to maximize drug recovery, and back-extraction into acid followed by reextraction to eliminate endogenous interferences. After evaporation, sample residues are derivatized with heptafluorobutyric anhydride and analyzed by separation on a fused-silica polymethylsiloxane capillary column and electron-capture detection. Calibration curves were linear in the ranges 0.1–2.0 μg/L and 2.0–20 μg/L, with within-run CVs of 9.7% at 0.1 μg/L to 5.0% at 20 μg/L, and total CVs of 15.9% at 0.1 μg/L to 6.5% at 10 μg/L. The limit of quantification was 0.1 μg/L. The method was utilized in studies to determine the absolute bioavailability of sublingual doses of 2 mg of buprenorphine in 1 mL of 300 mL/L ethanol and the bioequivalence of sublingual 8-mg tablet and 300 mL/L ethanol solution formulations.
- Subjects :
- Detection limit
Chromatography
Chromatography, Gas
Chemistry
Biochemistry (medical)
Clinical Biochemistry
Administration, Sublingual
Biological Availability
Bioequivalence
Sensitivity and Specificity
Dosage form
Sublingual administration
Buprenorphine
Analgesics, Opioid
Electron capture detector
Pharmacokinetics
Drug Stability
Injections, Intravenous
Humans
Gas chromatography
Quantitative analysis (chemistry)
Subjects
Details
- ISSN :
- 00099147
- Volume :
- 43
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Clinical chemistry
- Accession number :
- edsair.doi.dedup.....e612194604355df79af00d1411b6771d