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A simple modification of the Baermann method for diagnosis of strongyloidiasis
- Source :
- Memórias do Instituto Oswaldo Cruz., Vol 96, Iss 6, Pp 805-807 (2001), Memórias do Instituto Oswaldo Cruz, Volume: 96, Issue: 6, Pages: 805-807, Published: AUG 2001
- Publication Year :
- 2001
- Publisher :
- FapUNIFESP (SciELO), 2001.
-
Abstract
- The diagnosis of Strongyloides stercoralis infections is routinely made by microscopic observation of larvae in stool samples, a low sensitivity method, or by other, most effective methods, such as the Baermann or agar culture plate methods. We propose in this paper a practical modification of Baermann method. One hundred and six stool samples from alcoholic patients were analyzed using the direct smear test, agar culture plate method, the standard Baermann method, and its proposed modification. For this modification the funnel used in the original version of the method is substituted by a test tube with a rubber stopper, perforated to allow insertion of a pipette tip. The tube with a fecal suspension is inverted over another tube containing 6 ml of saline solution and incubated at 37 degrees C for at least 2 h. The saline solution from the second tube is centrifuged and the pellet is observed microscopically. Larva of S. stercoralis were detected in six samples (5.7%) by the two versions of the Baermann method. Five samples were positive using the agar culture plate method, and only in two samples the larva were observed using direct microscopic observation of fecal smears. Cysts of Endolimax nana and Entamoeba histolytica/dyspar were also detected in the modification of Baermann method. Data obtained by the modified Baermann method suggest that this methodology may helps concentrate larvae of S. stercoralis as efficiently as the original method.
- Subjects :
- Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
food.ingredient
lcsh:RC955-962
lcsh:QR1-502
intestinal parasites
Biology
direct smear
Sensitivity and Specificity
lcsh:Microbiology
Microbiology
Strongyloides stercoralis
Feces
food
agar culture plate method
Strongyloides
medicine
Animals
Humans
Agar
Plate method
Chromatography
medicine.disease
biology.organism_classification
Culture Media
Microscopic observation
Strongyloidiasis
Parasitology
Direct smear
Baermann method
Subjects
Details
- ISSN :
- 00740276
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz
- Accession number :
- edsair.doi.dedup.....e60ffd0ce7cb3b5984087c22e019d965