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Regulation of Molecular Pathways in Ischemia-Reperfusion Injury After Liver Transplantation
- Source :
- Transplantation. 96:926-934
- Publication Year :
- 2013
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2013.
-
Abstract
- Background Ischemia-reperfusion (I/R) injury is a multifactorial phenomenon that occurs during the transplant event and frequently compromises early graft function after liver transplantation (LT). Current comprehension of molecular mechanisms and regulation processes of I/R injury lacks clarity. MicroRNA (miRNA) regulation results critical in several biological processes. Methods This study evaluated gene expression and miRNA expression profiles using microarrays in 34 graft biopsies collected at preimplantation (L1) and at 90 min postreperfusion (L2) from consecutives deceased-donor LT recipients. miRNA profiles were first analyzed. Data integration analysis (gene expression/miRNA expression) aimed to identify potential target genes for each identified miRNA from the L1/L2 differential gene expression profile. Results Pairwise comparison analyses identified 40 miRNAs and 3168 significantly differentially expressed genes at postreperfusion time compared with preimplantation time. Pathway analysis of miRNAs associated these profiles with antiapoptosis, inhibition of cellular proliferation, and proinflammatory processes. Target analysis identified an miRNA-associated molecular profile of 2172 genes involved in cellular growth and proliferation modulation by cell cycle regulation, cell death and survival, and proinflammatory and anti-inflammatory processes. miRNA-independent genes involved proinflammatory molecules. Conclusion We identified a miRNA profile involved in posttranscriptional regulatory mechanisms in I/R injury post-LT. A better understanding of these molecular processes involved in I/R may contribute to develop new strategies to minimize graft injury.
- Subjects :
- Adult
Graft Rejection
Male
Transcription, Genetic
Biopsy
Biology
Bioinformatics
Article
Proinflammatory cytokine
Young Adult
microRNA
Gene expression
Humans
Postoperative Period
Gene
Aged
Regulation of gene expression
Transplantation
Gene Expression Profiling
Middle Aged
Cell cycle
Liver Transplantation
Gene expression profiling
MicroRNAs
Gene Expression Regulation
Reperfusion Injury
Cancer research
Female
DNA microarray
Follow-Up Studies
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....e601b71f1cf9c29dd82d73510d7fb64d