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Therapeutic effect of human adipose-derived stem cells and their secretome in experimental diabetic pain
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017), Scientific Reports
- Publication Year :
- 2017
-
Abstract
- Painful neuropathy is one of the complications of diabetes mellitus that adversely affects patients’quality of life. Pharmacological treatments are not fully satisfactory, and novel approaches needed. In a preclinical mouse model of diabetes the effect of both human mesenchymal stromal cells from adipose tissue (hASC) and their conditioned medium (hASC-CM) was evaluated. Diabetes was induced by streptozotocin. After neuropathic hypersensitivity was established, mice were intravenously injected with either 1 × 106 hASC or with CM derived from 2 × 106 hASC. Both hASC and CM (secretome) reversed mechanical, thermal allodynia and thermal hyperalgesia, with a rapid and long lasting effect, maintained up to 12 weeks after treatments. In nerves, dorsal root ganglia and spinal cord of neuropathic mice we determined high IL-1β, IL-6 and TNF-α and low IL-10 levels. Both treatments restored a correct pro/antinflammatory cytokine balance and prevented skin innervation loss. In spleens of streptozotocin-mice, both hASC and hASC-CM re-established Th1/Th2 balance that was shifted to Th1 during diabetes. Blood glucose levels were unaffected although diabetic animals regained weight, and kidney morphology was recovered by treatments. Our data show that hASC and hASC-CM treatments may be promising approaches for diabetic neuropathic pain, and suggest that cell effect is likely mediated by their secretome.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
lcsh:Medicine
Adipose tissue
Mice
Nerve Fibers
Diabetic Neuropathies
Ganglia, Spinal
CUTANEOUS INNERVATION
lcsh:Science
Kidney
Multidisciplinary
NEUROPATHIC PAIN
MOUSE MODEL
Cytokine
medicine.anatomical_structure
Adipose Tissue
Spinal Cord
PERIPHERAL NEUROPATHY
REGENERATION
Neuropathic pain
Cytokines
Inflammation Mediators
Ubiquitin Thiolesterase
medicine.drug
Calcitonin
medicine.medical_specialty
Mesenchymal Stem Cell Transplantation
Article
Diabetes Mellitus, Experimental
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Animals
Humans
Analysis of Variance
business.industry
lcsh:R
Therapeutic effect
Mesenchymal stem cell
Mesenchymal Stem Cells
Streptozotocin
medicine.disease
Disease Models, Animal
030104 developmental biology
Endocrinology
Culture Media, Conditioned
lcsh:Q
business
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017), Scientific Reports
- Accession number :
- edsair.doi.dedup.....e5ca448813ad4eb5fa359fae174c29d7