Back to Search Start Over

Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters

Authors :
Munish Puri
Serena L. Wilkens
Colin J. Barrow
Dilip Singh
Anshu S. Mathur
Deepak K. Tuli
Source :
Bioresource technology. 143
Publication Year :
2013

Abstract

A fast growing strain of Chlorella saccharophila was isolated from the marine water of New Zealand and grown in heterotrophic conditions using glucose or glycerol as a carbon source. Biomass production was found to be higher in culture fed with glucose (2.14±0.08 g L(-1)) as compared to glycerol (0.378±0.04 g L(-1)). Lipid accumulation was similar for both carbon sources, at approximately 22% of dry cell weight. However, carotenoid yield was higher with glycerol (0.406±0.0125 mg g(-1)) than with glucose (0.21±0.034 mg g(-1)). Further optimization of the growth of the isolate gave maximal carotenoid production of 16.39±1.19 mg g(-1) total carotenoid, containing 11.32±0.64 mg g(-1) zeaxanthin and 5.07±0.55 mg g(-1) β-carotene. Comparison of various chemical and physical carotenoid extraction methods showed that ultrasonication was required for maximum extraction yields. The new strain has potential for biofuel, with carotenoid co-production.

Details

ISSN :
18732976
Volume :
143
Database :
OpenAIRE
Journal :
Bioresource technology
Accession number :
edsair.doi.dedup.....e5bbe5f41594ddc2bd54ef755482171d