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JC virus infection of hematopoietic progenitor cells, primary B lymphocytes, and tonsillar stromal cells: implications for viral latency
- Source :
- Journal of Virology. 70:7004-7012
- Publication Year :
- 1996
- Publisher :
- American Society for Microbiology, 1996.
-
Abstract
- The human polyomavirus JC virus (JCV) infects myelin-producing cells in the central nervous system, resulting in the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). JCV-induced PML occurs most frequently in immunosuppressed individuals, with the highest incidence in human immunodeficiency type 1-infected patients, ranging between 4 and 6% of all AIDS cases. Although JCV targets a highly specialized cell in the central nervous system, infection is widespread, with more than 80% of the human population worldwide demonstrating serum antibodies. A number of clinical and laboratory studies have now linked the pathogenesis of PML with JCV infection in lymphoid cells. For example, JCV-infected lymphocytes have been suggested as possible carriers of virus to the brain following reactivation of a latent infection in lymphoid tissues. To further define the cellular tropism associated with JCV, we have attempted to infect immune system cells, including CD34+ hematopoietic progenitor cells derived from human fetal liver, primary human B lymphocytes, and human tonsillar stromal cells. Our results demonstrate that these cell types as well as a CD34+ human cell line, KG-1a, are susceptible to JCV infection. JCV cannot, however, infect KG-1, a CD34+ cell line which differentiates into a macrophage-like cell when treated with phorbol esters. In addition, peripheral blood B lymphocytes isolated by flow cytometry from a PML patient demonstrate JCV infection. These results provide direct evidence that JCV is not strictly neurotropic but can infect CD34+ hematopoietic progenitor cells and those cells which have differentiated into a lymphocytic, but not monocytic, lineage.
- Subjects :
- Stromal cell
viruses
Palatine Tonsil
Immunology
JC virus
CD34
Biology
medicine.disease_cause
Microbiology
Virus
Immune system
Virology
Virus latency
medicine
Humans
Cells, Cultured
B-Lymphocytes
Polyomavirus Infections
Progressive multifocal leukoencephalopathy
JC Virus Infection
virus diseases
Hematopoietic Stem Cells
medicine.disease
JC Virus
Virus Latency
Tumor Virus Infections
Organ Specificity
Insect Science
Stromal Cells
Research Article
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....e5b20e1e5d67d3def6fd81b4da07dea5