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Systemic eosinophil response induced by respiratory syncytial virus
- Source :
- Clinical and Experimental Immunology. 144:409-417
- Publication Year :
- 2006
- Publisher :
- Oxford University Press (OUP), 2006.
-
Abstract
- SummaryRespiratory syncytial virus (RSV) is a common cause of lower respiratory tract disease (LRTD) in infants. Eosinophils have been suggested to play a role in the disease pathogenesis of LRTD. Inflammation can induce functional and morphological alterations of peripheral blood granulocytes. In patients with RSV LRTD, we aimed to investigate the eosinophil activation status by analysing surface markers. In vitro stimulation of eosinophils with cytokines leads to up-regulation of CD11b and priming markers recognized by the recently developed priming markers A17 and A27, whereas interleukin (IL)-5Rα is being down-regulated. In 51 patients and 10 controls we examined the expression of these surface markers on eosinophils in moderate to severe RSV-induced LRTD patients at the time of admission and 6 weeks later during the convalescence phase. RSV-patients were characterized by a higher eosinophil CD11b expression compared to controls. Although basal A17 and A27 expression was not increased, we observed a significantly higher expression of these priming epitopes on N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated cells of RSV patients compared with cells of controls, indicative of prior in vivo priming. Furthermore, IL-5Rα expression was down-regulated on peripheral blood eosinophils of these patients. Follow-up blood samples showed normalization of all markers but CD11b, which was persistently increased. Utilizing cellular markers, we observed that peripheral blood eosinophils from infants with RSV LRTD are in a more activated state compared to eosinophils of controls, which normalizes only partially during convalescence.
- Subjects :
- Male
media_common.quotation_subject
Immunology
Down-Regulation
Priming (immunology)
Inflammation
Respiratory Syncytial Virus Infections
Granulocyte
Biology
Leukocyte Count
Interleukin-5 Receptor alpha Subunit
Clinical Studies
Eosinophil activation
medicine
Bronchiolitis, Viral
Humans
Immunology and Allergy
Respiratory system
Cells, Cultured
media_common
CD11b Antigen
Convalescence
Infant, Newborn
Oxygen Inhalation Therapy
Infant
Interleukin
Receptors, Interleukin
respiratory system
Eosinophil
Eosinophils
Hospitalization
N-Formylmethionine Leucyl-Phenylalanine
medicine.anatomical_structure
Respiratory Syncytial Virus, Human
Acute Disease
Female
medicine.symptom
Follow-Up Studies
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....e5af16edbfb9ae88b47a36ad0cba6e93