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Regulation by Neurotransmitter Receptors of Serotonergic or Catecholaminergic Neuronal Cell Differentiation
- Source :
- Journal of Biological Chemistry. 275:9186-9192
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- The murine F9-derived 1C11 clone exhibits a stable epithelial morphology, expresses nestin, an early neuroectodermal marker, and expresses genes involved in neuroectodermal cell fate. Upon appropriate induction, 100% of 1C11 precursor cells develop neurite extensions and acquire neuronal markers (N-CAM, synaptophysin, gammagamma-enolase, and neurofilament) as well as the general functions of either serotonergic (1C11*(/5HT)) (5HT, 5-hydroxytryptamine) or noradrenergic (1C11**(/NE)) (NE, norepinephrine) neurons. The two programs are shown to be mutually exclusive. 1C11 thus behaves as a neuroepithelial cell line with a dual bioaminergic fate. 1C11*(/5HT) cells implement a functional 5-HT transporter and thereby a complete serotonergic phenotype within 4 days, whereas 5-HT(1B/D), 5-HT(2B), and 5-HT(2A) receptors are sequentially induced. The accurate time schedule of catecholaminergic differentiation was defined. Catecholamine synthesis, storage, and catabolism are acquired within 4 days; the noradrenergic phenotype is complete at day 12 and includes a functional norepinephrine transporter and an alpha(1D)-adrenoreceptor (day 8). The time-dependent onset of neurotransmitter-associated functions proper to either program is similar to in vivo observations. Along each pathway, the selective induction of serotonergic or adrenergic receptors is shown to be an essential part of the differentiation program, since they promote an autoregulation of the corresponding phenotype.
- Subjects :
- Serotonin
medicine.medical_specialty
Neurite
Cellular differentiation
Down-Regulation
Neuroectodermal Cell
Serotonergic
Biochemistry
Cell Line
Norepinephrine
Neurotransmitter receptor
Receptors, Adrenergic, alpha-1
Internal medicine
medicine
Animals
Molecular Biology
Neurons
Catecholaminergic
biology
Cell Differentiation
Cell Biology
Receptors, Adrenergic
Cell biology
Phenotype
Endocrinology
Norepinephrine transporter
Receptors, Serotonin
biology.protein
medicine.drug
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 275
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....e5a4ce9add17d042c44dd5bdbb648c8d