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Evaluation of a cuticle protein gene as a potential RNAi target in aphids
- Source :
- Pest Management Science. 76:134-140
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background RNA interference (RNAi) has potential as a pest insect control technique. One possible RNAi target is the cuticle protein, which is important in insect molting and development. As an example, here we evaluate the possibility of designing double-stranded RNA (RNA) that is effective for silencing the cuticle protein 19 gene (CP19) in aphids but is harmless to non-target predator insects. Results The sequences of CP19s were similar (86.6-94.4%) among the tested aphid species (Aphis citricidus, Acyrthosiphon pisum, and Myzus persicae) but different in the predator Propylaea japonica. Ingestion of species-specific dsRNAs of CP19 by the three aphids produced 39.3-64.2% gene silencing and 45.8-55.8% mortality. Ingestion of non-species-specific dsRNA (dsAcCP19) by Ac. pisum and M. persicae gave gene silencing levels ranging from 40.4% to 50.3% and 43.3-50.8% mortality. The dsApCP19 did not affect PjCP19 expression or developmental duration in P. japonica. Conclusion The results demonstrate that CP19 is a promising RNAi target for aphid control via one dsRNA design. The targeting of genes that are conserved in insect pests but not present in beneficial insects is a useful RNAi-based pest control strategy. © 2019 Society of Chemical Industry.
- Subjects :
- 0106 biological sciences
Insect Control
01 natural sciences
RNA interference
Animals
Gene silencing
Beneficial insects
Gene Silencing
Gene
RNA, Double-Stranded
Genetics
Aphid
biology
fungi
food and beverages
General Medicine
biology.organism_classification
Acyrthosiphon pisum
010602 entomology
RNA silencing
Aphids
Insect Science
RNA Interference
Myzus persicae
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15264998 and 1526498X
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Pest Management Science
- Accession number :
- edsair.doi.dedup.....e58506ba0233955430db0d850c0e089f