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Downregulation of Umbilical Cord Blood Levels of miR-374a in Neonatal Hypoxic Ischemic Encephalopathy
- Source :
- The Journal of pediatrics. 167(2)
- Publication Year :
- 2014
-
Abstract
- Objective To investigate the expression profile of microRNA (miRNA) in umbilical cord blood from infants with hypoxic ischemic encephalopathy (HIE). Study design Full-term infants with perinatal asphyxia were identified under strict enrollment criteria. Degree of encephalopathy was defined using both continuous multichannel electroencephalogram in the first 24 hours of life and modified Sarnat score. Seventy infants (18 controls, 33 with perinatal asphyxia without HIE, and 19 infants with HIE [further graded as 13 mild, 2 moderate, and 4 severe]) were included in the study. MiRNA expression profiles were determined using a microarray assay and confirmed using quantitative real-time polymerase chain reaction. Results Seventy miRNAs were differentially expressed between case and control groups. Of these hsa-miR-374a was the most significantly downregulated in infants with HIE vs controls. Validation of hsa-miR-374a expression using quantitative real-time polymerase chain reaction confirmed a significant reduction in expression among infants with HIE compared with those with perinatal asphyxia and healthy controls (mean relative quantification [SD] = 0.52 [0.37] vs 1.10 [1.52] vs 1.76 [1.69], P Conclusions We have shown a significant step-wise downregulation of hsa-miR-374a expression in cord blood of infants with perinatal asphyxia and subsequent HIE.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Encephalopathy
Down-Regulation
Real-Time Polymerase Chain Reaction
Gastroenterology
Umbilical cord
Severity of Illness Index
Hypoxic Ischemic Encephalopathy
Pregnancy
Internal medicine
Medicine
Humans
Prospective Studies
Prospective cohort study
Asphyxia Neonatorum
business.industry
Infant, Newborn
Infant
Electroencephalography
medicine.disease
Fetal Blood
Microarray Analysis
Perinatal asphyxia
MicroRNAs
Real-time polymerase chain reaction
medicine.anatomical_structure
Cord blood
Pediatrics, Perinatology and Child Health
Hypoxia-Ischemia, Brain
Female
business
Subjects
Details
- ISSN :
- 10976833
- Volume :
- 167
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of pediatrics
- Accession number :
- edsair.doi.dedup.....e5811525354c50d1666c0f7f005eb9f3