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Hepatoprotective effect and metabonomics studies of radix gentianae in rats with acute liver injury
- Source :
- Pharmaceutical Biology, article-version (VoR) Version of Record, Pharmaceutical Biology, Vol 59, Iss 1, Pp 1172-1180 (2021)
- Publication Year :
- 2021
-
Abstract
- Context As a well-known traditional Chinese medicine for protecting the liver, the mechanism of Radix Gentianae (RG) remains unclear. Objective The hepatoprotective effect and metabonomics of RG were studied to explore the molecular and metabolic mechanisms of RG protecting the liver. Materials and methods Sprague-Dawley rats were divided into control and model group (n = 10, orally given distilled water), intervention group (4 subgroups, n = 10, prophylactically and orally given 0.63, 2.5 and 5.6 g/kg RG and 0.2 g/kg bifendatatum for 7 d). On day 7 of the intervention, all rats except the control were injected intraperitoneally with 2.5% carbon tetrachloride vegetable oil solution (1.5 mL/kg) to induce liver injury. After 24 h of carbon tetrachloride injection, rat serum and liver tissue were collected for determining AST, ALT, TNF-α, MCP-1, IL-6, SOD, MDA, GSH, and GSH-PX. Rat serum was used for analysing endogenous metabolism by UPLC-Q-TOF-MS. Results Different doses of RG can significantly decrease the levels of AST, ALT, TNF-α, MCP-1, IL-6 and MDA, and increase the levels of SOD, GSH, and GSH-PX in rats with liver injury (p
- Subjects :
- Male
Pharmaceutical Science
Administration, Oral
Context (language use)
RM1-950
Traditional Chinese medicine
Steroid biosynthesis
Pharmacology
Protective Agents
Rats, Sprague-Dawley
chemistry.chemical_compound
Drug Discovery
medicine
pharmacodynamics
Animals
Metabolomics
Gentiana
Carbon Tetrachloride
Liver injury
Dose-Response Relationship, Drug
business.industry
Plant Extracts
Liver Diseases
General Medicine
Glutathione
medicine.disease
metabolic mechanism
Rats
Metabolic pathway
Disease Models, Animal
Complementary and alternative medicine
chemistry
Pharmacodynamics
Carbon tetrachloride
Molecular Medicine
Therapeutics. Pharmacology
molecular mechanism
business
Biomarkers
Research Article
Subjects
Details
- ISSN :
- 17445116
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical biology
- Accession number :
- edsair.doi.dedup.....e5666c6c5532188e1eeeaf62ea257543