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Cathepsin B-Responsive Liposomes for Controlled Anticancer Drug Delivery in Hep G2 Cells
- Source :
- Pharmaceutics, Volume 12, Issue 9, Pharmaceutics, Vol 12, Iss 876, p 876 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- In recent decades, several types of anticancer drugs that inhibit cancer cell growth and cause cell death have been developed for chemotherapeutic application. However, these agents are usually associated with side effects resulting from nonspecific delivery, which may induce cytotoxicity in healthy cells. To reduce the nonspecific delivery issue, nanoparticles have been successfully used for the delivery of anticancer drugs to specific target sites. In this study, a functional polymeric lipid, PEG-GLFG-K(C16)2 (PEG-GLFG, polyethylene glycol-Gly-Leu-Phe-Gly-Lys(C16)2), was synthesized to enable controlled anticancer drug delivery using cathepsin B enzyme-responsive liposomes. The liposomes composed of PEG-GLFG/DOTAP (1,2-dioleoyl-3-trimethylammonium-propane (chloride salt))/DPPC (dipalmitoylphosphatidylcholine)/cholesterol were prepared and characterized at various ratios. The GLFG liposomes formed were stable liposomes and were degraded when acted upon by cathepsin B enzyme. Doxorubicin (Dox) loaded GLFG liposomes (GLFG/Dox) were observed to exert an effective anticancer effect on Hep G2 cells in vitro and inhibit cancer cell proliferation in a zebrafish model.
- Subjects :
- GLFG peptide
cathepsin B
lcsh:RS1-441
Pharmaceutical Science
02 engineering and technology
Pharmacology
Article
Cathepsin B
lcsh:Pharmacy and materia medica
03 medical and health sciences
medicine
Doxorubicin
Cytotoxicity
030304 developmental biology
0303 health sciences
Liposome
Chemistry
technology, industry, and agriculture
021001 nanoscience & nanotechnology
In vitro
Hep G2
drug delivery
liposome
Drug delivery
Cancer cell
lipids (amino acids, peptides, and proteins)
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 19994923
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Pharmaceutics
- Accession number :
- edsair.doi.dedup.....e5620fed32268c388bd33c898dac2448