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β-Glucan-Induced IL-10 Secretion by Monocytes Triggers Porcine NK Cell Cytotoxicity
- Source :
- Frontiers in Immunology, Vol 12 (2021), Frontiers in Immunology, FRONTIERS IN IMMUNOLOGY
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- Beta-glucans are naturally occurring polysaccharides present in cell walls of fungi, yeast, bacteria, cereals, seaweed, and algae. These microbe-associated molecular patterns (MAMPs) possess immunomodulatory properties. In human, it has been suggested that NK cells can be activated by β-glucans. Here, we aimed to elucidate whether β-glucans modulate porcine NK cell responses in vitro and if so, how these effects are mediated. We investigated the effect of two β-glucans, Macrogard and Curdlan, which differ in solubility and structure. Direct addition of β-glucans to purified porcine NK cells did not affect cytotoxicity of these cells against K562 target cells. However, when using PBMC instead of purified NK cells, β-glucan addition significantly increased NK cell-mediated cytotoxicity. This effect depended on factors secreted by CD14+ monocytes upon β-glucan priming. Further analysis showed that monocytes secrete TNF-α, IL-6, and IL-10 upon β-glucan addition. Of these, IL-10 turned out to play a critical role in β-glucan-triggered NK cell cytotoxicity, since depletion of IL-10 completely abrogated the β-glucan-induced increase in cytotoxicity. Furthermore, addition of recombinant IL-10 to purified NK cells was sufficient to enhance cytotoxicity. In conclusion, we show that β-glucans trigger IL-10 secretion by porcine monocytes, which in turn leads to increased NK cell cytotoxicity, and thereby identify IL-10 as a potent stimulus of porcine NK cell cytotoxicity.
- Subjects :
- Cytotoxicity, Immunologic
lcsh:Immunologic diseases. Allergy
0301 basic medicine
beta-Glucans
CD14
Sus scrofa
Immunology
Cell
NK cells
Peripheral blood mononuclear cell
Monocytes
03 medical and health sciences
0302 clinical medicine
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Paracrine Communication
medicine
Animals
Humans
Immunology and Allergy
Secretion
Veterinary Sciences
Cytotoxicity
glucans
Original Research
Secretory Pathway
immune modulation
β
Chemistry
Monocyte
Biology and Life Sciences
macrogard
Coculture Techniques
Interleukin-10
Cell biology
Killer Cells, Natural
Interleukin 10
030104 developmental biology
medicine.anatomical_structure
monocyte
IL-10
β-glucans
curdlan
K562 Cells
lcsh:RC581-607
030215 immunology
K562 cells
Subjects
Details
- ISSN :
- 16643224
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....e56047e8fb7d4ab7dfcc96a1e68163c6