Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:57:50Z No. of bitstreams: 1 S0102-695X2012000200003.pdf: 824135 bytes, checksum: 025b07c2b5fb8d21d0ff45ee59aa142b (MD5) Made available in DSpace on 2013-08-22T18:57:50Z (GMT). No. of bitstreams: 1 S0102-695X2012000200003.pdf: 824135 bytes, checksum: 025b07c2b5fb8d21d0ff45ee59aa142b (MD5) Previous issue date: 2012-04-01 Made available in DSpace on 2013-09-30T19:52:56Z (GMT). No. of bitstreams: 2 S0102-695X2012000200003.pdf: 824135 bytes, checksum: 025b07c2b5fb8d21d0ff45ee59aa142b (MD5) S0102-695X2012000200003.pdf.txt: 50825 bytes, checksum: 16e60e550abb63439cb5f12306d4aaac (MD5) Previous issue date: 2012-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:56:10Z No. of bitstreams: 2 S0102-695X2012000200003.pdf: 824135 bytes, checksum: 025b07c2b5fb8d21d0ff45ee59aa142b (MD5) S0102-695X2012000200003.pdf.txt: 50825 bytes, checksum: 16e60e550abb63439cb5f12306d4aaac (MD5) Made available in DSpace on 2014-05-20T13:56:10Z (GMT). No. of bitstreams: 2 S0102-695X2012000200003.pdf: 824135 bytes, checksum: 025b07c2b5fb8d21d0ff45ee59aa142b (MD5) S0102-695X2012000200003.pdf.txt: 50825 bytes, checksum: 16e60e550abb63439cb5f12306d4aaac (MD5) Previous issue date: 2012-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K. Universidade de São Paulo Instituto de Química de São Carlos Universidade Federal de São Carlos (UFSCar) Departamento de Ecologia e Biologia Evolutiva Universidade Federal do Ceará (UFC) Departamento de Fisiologia e Farmacologia Universidade Estadual de Campinas Faculdade de Odontologia de Piracicaba Departamento de Diagnóstico Oral Universidade Estadual Paulista Departamento de Bioquímica e Microbiologia Universidade Estadual Paulista Departamento de Bioquímica e Microbiologia FAPESP: 10/50190-2 FAPESP: 05/60175-2