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Dynamic proteomic and metabonomic analysis reveal dysfunction and subclinical injury in rat liver during restraint stress
- Source :
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1794:1751-1765
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Stress is a risk factor for many diseases. In this study, we used fluorescence difference gel electrophoresis combined MALDI-TOF/TOF and 1H-NMR to monitor the intracellular processes in rat liver at proteomic and metabonomic levels when a rat was treated with restraint stress for 8 weeks. Dynamic changes in 42 proteins and 32 chemical groups were monitored and identified. These proteins and chemical groups were implicated in glycolysis, the tricarboxylic acid cycle, fatty acid oxidation, and the urea cycle. To verify the DIGE result, three proteins including DJ-1, Blvrb and AdoHycase were validated by Western blot. Furthermore, some metabolites related to diseases such as lactate, fatty acid, glucose and homocysteine, were observed to be increasing during 8 weeks of restraint stress. Our data indicated that subclinical hepatic injury occurs during restraint stress, including inhibition of glycolysis and gluconeogenesis in the liver, and dysfunction of fatty acid beta-oxidation. The results suggest a comprehensive map that addresses how functional proteins act on metabolites to produce energy and process materials in rat liver as it responds to restraint stress. Further functional study on these dynamic change proteins and metabolites may lead to better understanding of the mechanisms of stress-induced diseases.
- Subjects :
- Male
Restraint, Physical
medicine.medical_specialty
Magnetic Resonance Spectroscopy
Time Factors
Proteome
Difference gel electrophoresis
Blotting, Western
Molecular Sequence Data
Biophysics
Biology
Models, Biological
Biochemistry
Analytical Chemistry
Stress, Physiological
Internal medicine
medicine
Metabolome
Animals
Electrophoresis, Gel, Two-Dimensional
Glycolysis
Amino Acid Sequence
Rats, Wistar
Homocysteine
Molecular Biology
Beta oxidation
chemistry.chemical_classification
Fatty acid
Lipid Metabolism
Rats
Citric acid cycle
Endocrinology
Liver
Gluconeogenesis
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Urea cycle
Carbohydrate Metabolism
Subjects
Details
- ISSN :
- 15709639
- Volume :
- 1794
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- Accession number :
- edsair.doi.dedup.....e53d6e64080ac5b4e56c0fc4f0d584b0