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Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells
- Source :
- Biochemical and biophysical research communications. 480(3)
- Publication Year :
- 2016
-
Abstract
- Activin A (Act A), a member of the transforming growth factor-beta (TGF-β), reduces neuronal apoptosis during cerebral ischemia through Act A/Smads signaling pathway. However, little is known about the effect of Act A/Smads pathway on autophagy in neurons. Here, we found that oxygen-glucose deprivation (OGD)-induced autophagy was suppressed by exogenous Act A in a concentration-dependent manner and enhanced by Act A/Smads pathway inhibitor (ActRIIA-Ab) in neuronal PC12 cells. These results indicate that Act A/Smads pathway negatively regulates autophagy in OGD-treated PC12 cells. In addition, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways are involved in the OGD-induced autophagy. The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.
- Subjects :
- 0301 basic medicine
Cell Survival
MAP Kinase Signaling System
p38 mitogen-activated protein kinases
Biophysics
Ischemia
Smad Proteins
Biochemistry
PC12 Cells
p38 Mitogen-Activated Protein Kinases
Cell Line
03 medical and health sciences
0302 clinical medicine
Stress, Physiological
medicine
Autophagy
Animals
Molecular Biology
Neuronal apoptosis
Inhibin-beta Subunits
Neurons
Kinase
Chemistry
Cell Biology
medicine.disease
Cell biology
Rats
Activin a
Oxygen
Oxidative Stress
030104 developmental biology
nervous system
Oxygen glucose deprivation
Signal transduction
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 480
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....e53922f8b394424e350d71dbe4e2f4df