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Comparison of three DNA extraction methods for molecular confirmation of Mycobacterium avium subspecies paratuberculosis from the VersaTrek™ liquid cultures of bovine fecal samples

Authors :
Willard Feria
Deepanker Tewari
Nagaraja R. Thirumalapura
Source :
Journal of microbiological methods. 152
Publication Year :
2018

Abstract

We evaluated three DNA extraction methods for confirmation of Mycobacterium avium subspecies paratuberculosis from liquid cultures of bovine feces. Use of DNA Extract All Reagents Kit™ resulted in efficient extraction of amplifiable DNA from higher proportion (96.29%) of known positive samples compared to Chelex-100 resin (25.92%) and polyethylene glycol (0%).

Details

ISSN :
18728359
Volume :
152
Database :
OpenAIRE
Journal :
Journal of microbiological methods
Accession number :
edsair.doi.dedup.....e538a6e1bd0ca690f410ab8e95d1d8ca