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OxyR Is a Convergent Target for Mutations Acquired during Adaptation to Oxidative Stress-Prone Metabolic States
- Source :
- Molecular Biology and Evolution, Anand, A, Chen, K, Catoiu, E, Sastry, A V, Olson, C A, Sandberg, T E, Seif, Y, Xu, S, Szubin, R, Yang, L, Feist, A M & Palsson, B O 2020, ' OxyR Is a Convergent Target for Mutations Acquired during Adaptation to Oxidative Stress-Prone Metabolic States ', Molecular Biology and Evolution, vol. 37, no. 3, pp. 660-666 . https://doi.org/10.1093/molbev/msz251
- Publication Year :
- 2019
- Publisher :
- Oxford University Press, 2019.
-
Abstract
- Oxidative stress is concomitant with aerobic metabolism. Thus, bacterial genomes encode elaborate mechanisms to achieve redox homeostasis. Here we report that the peroxide-sensing transcription factor, oxyR, is a common mutational target using bacterial species belonging to two genera, Escherichia coli and Vibrio natriegens, in separate growth conditions implemented during laboratory evolution. The mutations clustered in the redox active site, dimer interface, and flexible redox loop of the protein. These mutations favor the oxidized conformation of OxyR that results in constitutive expression of the genes it regulates. Independent component analysis of the transcriptome revealed that the constitutive activity of OxyR reduces DNA damage from reactive oxygen species, as inferred from the activity of the SOS response regulator LexA. This adaptation to peroxide stress came at a cost of lower growth, as revealed by calculations of proteome allocation using genome-scale models of metabolism and macromolecular expression. Further, identification of similar sequence changes in natural isolates of E. coli indicates that adaptation to oxidative stress through genetic changes in oxyR can be a common occurrence.
- Subjects :
- Models, Molecular
DNA damage
Protein Conformation
Biology
Vibrio natriegens
medicine.disease_cause
Transcriptome
03 medical and health sciences
Bacterial Proteins
Catalytic Domain
Genetics
medicine
Escherichia coli
SOS response
Molecular Biology
Gene
Ecology, Evolution, Behavior and Systematics
Discoveries
030304 developmental biology
adaptive laboratory evolution
Vibrio
0303 health sciences
030306 microbiology
Escherichia coli Proteins
systems biology
Gene Expression Regulation, Bacterial
biology.organism_classification
Adaptation, Physiological
Repressor Proteins
Oxidative Stress
Oxidative stress
Mutation
bacteria
Repressor lexA
Directed Molecular Evolution
Adaptive laboratory evolution
Systems biology
Reactive Oxygen Species
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 15371719 and 07374038
- Volume :
- 37
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular Biology and Evolution
- Accession number :
- edsair.doi.dedup.....e512f285311bf32bd74d85862145278d