Back to Search
Start Over
Correlation of [18F]FMISO autoradiography and pimonodazole immunohistochemistry in human head and neck carcinoma xenografts
- Source :
- European Journal of Nuclear Medicine and Molecular Imaging, 35, 10, pp. 1803-11, European Journal of Nuclear Medicine and Molecular Imaging, 35, 1803-11
- Publication Year :
- 2008
-
Abstract
- Contains fulltext : 70698.pdf (Publisher’s version ) (Closed access) PURPOSE: Tumour cell hypoxia is a common feature in solid tumours adversely affecting radiosensitivity and chemosensitivity in head and neck squamous cell carcinomas. Positron emission tomography (PET) using the tracer [(18)F]fluoromisonidazole ([(18)F]FMISO) is most frequently used for non-invasive evaluation of hypoxia in human tumours. A series of ten human head and neck xenograft tumour lines was used to validate [(18)F]FMISO as hypoxia marker at the microregional level. METHODS: Autoradiography after injection of [(18)F]FMISO was compared with immunohistochemical staining for the hypoxic cell marker pimonidazole in the same tumour sections of ten different human head and neck xenograft tumour lines. The methods were compared: first, qualitatively considering the microarchitecture; second, by obtaining a pixel-by-pixel correlation of both markers at the microregional level; third, by measuring the signal intensity of both images; and fourth, by calculating the hypoxic fractions by pimonidazole labelling. RESULTS: The pattern of [(18)F]FMISO signal was dependent on the distribution of hypoxia at the microregional level. The comparison of [(18)F]FMISO autoradiography and pimonidazole immunohistochemistry by pixel-by-pixel analysis revealed moderate correlations. In five tumour lines, a significant correlation between the mean [(18)F]FMISO and pimonidazole signal intensity was found (range, r(2)=0.91 to r(2)=0.99). Comparison of the tumour lines with respect to the microregional distribution pattern of hypoxia revealed that the correlation between the mean signal intensities strongly depended on the microarchitecture. Overall, a weak but significant correlation between hypoxic fractions based on pimonidazole labeling and the mean [(18)F]FMISO signal intensity was observed (r(2)=0.18, p=0.02). For the three tumour models with a ribbon-like microregional distribution pattern of hypoxia, the correlation between the hypoxic fraction and the mean [(18)F]FMISO signal intensity was much stronger and more significant (r(2)=0.73, p
- Subjects :
- 18F-Fluoromisonidazole
Pathology
medicine.medical_specialty
Mice, Nude
Aetiology, screening and detection [ONCOL 5]
Sensitivity and Specificity
Mice
Immune Regulation [NCMLS 2]
Translational research [ONCOL 3]
Biomarkers, Tumor
medicine
Carcinoma
Animals
Humans
Pimonidazole
Radiology, Nuclear Medicine and imaging
Radiosensitivity
Misonidazole
medicine.diagnostic_test
business.industry
Chemistry
Reproducibility of Results
Immunotherapy, gene therapy and transplantation [UMCN 1.4]
Functional imaging [IGMD 1]
General Medicine
Hypoxia (medical)
medicine.disease
Pathogenesis and modulation of inflammation [N4i 1]
Head and Neck Neoplasms
Nitroimidazoles
Positron emission tomography
Autoradiography
Immunohistochemistry
Functional Imaging [UMCN 1.1]
Radiopharmaceuticals
medicine.symptom
Nuclear medicine
business
FMISO
Subjects
Details
- ISSN :
- 16197070
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- European Journal of Nuclear Medicine and Molecular Imaging
- Accession number :
- edsair.doi.dedup.....e506fdc78fde1d926a6ee2c7c0c25642