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The occurrence of methicillin-resistant non-aureus staphylococci in samples from cows, young stock, and the environment on German dairy farms
- Source :
- Journal of Dairy Science. 104:4604-4614
- Publication Year :
- 2021
- Publisher :
- American Dairy Science Association, 2021.
-
Abstract
- This study aimed to determine the occurrence of methicillin-resistant (MR) non-aureus staphylococci (NAS) on 20 preselected German dairy farms. Farms were selected based on the detection of methicillin-resistant Staphylococcus aureus (MRSA) during previous diagnostic investigations. Bacterial culture of presumptive MR-NAS was based on a 2-step enrichment method that has been recommended for MRSA detection. Quarter milk samples (QMS), bulk tank milk, swab samples from young stock, and environmental samples were collected for bacterial culture. Methicillin-resistant NAS were detected on all study farms. The MR-NAS positive test rate was 3.3% (77/2,347) in QMS, 42.1% (8/19) in bulk tank milk, 29.1% (59/203) in nasal swabs from milk-fed calves, 18.3% (35/191) in postweaning calves, and 7.3% (14/191) in nasal swabs from prefresh heifers. In the environment, MR-NAS were detected in dust samples on 25% (5/20) of the dairy farms as well as in teat liners and suckers from automatic calf feeders. The geometric mean somatic cell count in QMS affected by MR-NAS (183,000 cells/mL) was slightly higher compared with all QMS (114,000 cells/mL). Nine MR-NAS species were identified; Staph. sciuri, Staph. lentus, Staph. fleurettii, Staph. epidermidis, and Staph. haemolyticus were the most common species. In addition, 170 NAS isolates were identified that showed reduced cefoxitin susceptibility (4 mg/L) but did not harbor the mecA or mecC genes. On some farms, similar mobile genetic elements were detected in MR-NAS and MRSA. It was suggested that resistance genes may be transferred between NAS and Staph. aureus on the respective farms.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Veterinary medicine
Farms
Microbiological culture
Staphylococcus
Cattle Diseases
Microbial Sensitivity Tests
Biology
medicine.disease_cause
03 medical and health sciences
Antibiotic resistance
Genetics
medicine
Animals
Bulk tank
Cefoxitin
Dairy cattle
030304 developmental biology
0303 health sciences
0402 animal and dairy science
food and beverages
04 agricultural and veterinary sciences
Staphylococcal Infections
bacterial infections and mycoses
040201 dairy & animal science
Milk
Staphylococcus aureus
Nasal Swab
Cattle
Female
Methicillin Resistance
Animal Science and Zoology
Somatic cell count
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 00220302
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Journal of Dairy Science
- Accession number :
- edsair.doi.dedup.....e4f9507bcf90fc0a4414c455ca0bcba9