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Alternative sigma factor σE has an important role in stress tolerance of Yersinia pseudotuberculosis IP32953
- Source :
- Applied and environmental microbiology. 79(19)
- Publication Year :
- 2013
-
Abstract
- Yersinia pseudotuberculosis is an important pathogen that probably survives well in the modern food chain. However, little is known about the mechanisms that allow the growth of this pathogen in foods under stress conditions. The expression of rpoE encoding σ E was defined by quantitative real-time reverse transcription-PCR. Expression of rpoE was induced at 3°C, 37°C, and 42°C, under exposure to 3% NaCl, 3% ethanol, or high and low pH, in relation to its expression at the optimum growth temperature of 28°C of Y. pseudotuberculosis . Mutation of rpoE either impaired or abolished growth under stresses caused by low or high temperature, low pH, and ethanol. In addition, the growth temperature range of the mutant was significantly diminished compared to that of the wild-type strain IP32953. The results were confirmed with complementation of the mutant. Thus, σ E plays a significant role in the stress tolerance of Y. pseudotuberculosis IP32953 and probably contributes to the survival of this pathogen in the food chain.
- Subjects :
- Mutant
Sigma Factor
Biology
Sodium Chloride
Real-Time Polymerase Chain Reaction
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Sigma factor
Stress, Physiological
Yersinia pseudotuberculosis
Ethanol metabolism
Pathogen
030304 developmental biology
2. Zero hunger
Regulation of gene expression
0303 health sciences
Microbial Viability
Ecology
Strain (chemistry)
Ethanol
030306 microbiology
Gene Expression Profiling
Genetic Complementation Test
Temperature
Gene Expression Regulation, Bacterial
Hydrogen-Ion Concentration
biology.organism_classification
Complementation
Food Microbiology
Gene Deletion
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336
- Volume :
- 79
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....e4f57cab5fbe5193c2a3fd6658c59bf2