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Reelin Signaling Facilitates Maturation of CA1 Glutamatergic Synapses
- Source :
- Journal of Neurophysiology. 97:2312-2321
- Publication Year :
- 2007
- Publisher :
- American Physiological Society, 2007.
-
Abstract
- Reelin signaling through the low-density lipoprotein receptor family members, apoliproprotein E receptor 2 (apoER2) and very-low-density lipoprotein receptor (VLDLR), plays a pivotal role in dictating neuronal lamination during embryonic brain development. Recent evidence suggests that this signaling system also plays a role in the postnatal brain to modulate synaptic transmission, plasticity, and cognitive behavior, mostly likely due to a functional coupling with N-methyl-d-aspartate (NMDA) receptors. In this study, we investigated the effects of reelin on the maturation of CA1 glutamatergic function using electrophysiological and biochemical approaches. In cultured hippocampal slices, reelin treatment increased the amplitude of AMPAR-mediated miniature excitatory postsynaptic currents and the evoked AMPA/NMDA receptor current ratios. In addition, reelin treatment also reduced the number of silent synapses, facilitated a developmental switch from NR2B to NR2A of NMDARs, and increased surface expression of AMPARs in CA1 tissue. In cultured hippocampal neurons from reeler embryos, reduced numbers of AMPAR subunit GluR1 and NMDAR subunit NR1 clustering were observed compared with those obtained from wild-type embryos. Supplementing reelin in the reeler culture obliterated these genotypic differences. These results demonstrate that reelin- and lipoprotein receptor-mediated signaling may operate during developmental maturation of hippocampal glutamatergic function and thus represent a potential important mechanism for controlling synaptic strength and plasticity in the postnatal hippocampus.
- Subjects :
- Physiology
Cell Adhesion Molecules, Neuronal
Glutamic Acid
Mice, Transgenic
Nerve Tissue Proteins
Transfection
Hippocampus
Mice
Glutamatergic
Animals
Humans
Reelin
Receptor
Cells, Cultured
6-Cyano-7-nitroquinoxaline-2,3-dione
Analysis of Variance
Extracellular Matrix Proteins
biology
Pyramidal Cells
musculoskeletal, neural, and ocular physiology
General Neuroscience
Serine Endopeptidases
Excitatory Postsynaptic Potentials
Gene Expression Regulation, Developmental
Dose-Response Relationship, Radiation
DAB1
Electric Stimulation
Reelin Protein
2-Amino-5-phosphonovalerate
Animals, Newborn
nervous system
Synapses
biology.protein
Excitatory Amino Acid Antagonists
Neuroscience
Signal Transduction
Lipoprotein
Subjects
Details
- ISSN :
- 15221598 and 00223077
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of Neurophysiology
- Accession number :
- edsair.doi.dedup.....e4c85f6320357a32048ad25d54f320e2