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CD133 Expression Defines a Tumor Initiating Cell Population in Primary Human Ovarian Cancer
- Source :
- Stem Cells. 27:2875-2883
- Publication Year :
- 2009
- Publisher :
- Oxford University Press (OUP), 2009.
-
Abstract
- Evidence is accumulating that solid tumors contain a rare phenotypically distinct population of cells, termed cancer stem cells (CSC), which give rise to and maintain the bulk of the tumor. These CSC are thought to be resistant to current chemotherapeutic strategies due to their intrinsic stem-like properties and thus may provide the principal driving force behind recurrent tumor growth. Given the high frequency of recurrent metastasis associated with human ovarian cancer, we sought to determine whether primary human ovarian tumors contain populations of cells with enhanced tumor-initiating capacity, a characteristic of CSC. Using an in vivo serial transplantation model, we show that primary uncultured human ovarian tumors can be reliably propagated in NOD/SCID mice, generating heterogeneous tumors that maintain the histological integrity of the parental tumor. The observed frequency of tumor engraftment suggests only certain subpopulations of ovarian tumor cells have the capacity to recapitulate tumor growth. Further profiling of human ovarian tumors for expression of candidate CSC surface markers indicated consistent expression of CD133. To determine whether CD133 expression could define a tumor-initiating cell population in primary human ovarian tumors, fluorescence-activated cell sorting (FACS) methods were employed. Injection of sorted CD133+ and CD133− cell populations into NOD/SCID mice established that tumor-derived CD133+ cells have an increased tumorigenic capacity and are capable of recapitulating the original heterogeneous tumor. Our data indicate that CD133 expression defines a NOD/SCID tumor initiating subpopulation of cells in human ovarian cancer that may be an important target for new chemotherapeutic strategies aimed at eliminating ovarian cancer. Disclosure of potential conflicts of interest is found at the end of this article.
- Subjects :
- Population
Cell
Cell Count
Mice, SCID
Nod
Biology
Metastasis
Mice
Ovarian tumor
Antigens, CD
Mice, Inbred NOD
Cancer stem cell
Biomarkers, Tumor
medicine
Animals
Humans
AC133 Antigen
education
Glycoproteins
Ovarian Neoplasms
education.field_of_study
Cell Biology
Cell sorting
medicine.disease
Xenograft Model Antitumor Assays
medicine.anatomical_structure
Immunology
Neoplastic Stem Cells
Cancer research
Molecular Medicine
Female
Peptides
Ovarian cancer
Developmental Biology
Subjects
Details
- ISSN :
- 15494918 and 10665099
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Stem Cells
- Accession number :
- edsair.doi.dedup.....e4c3d6eb42d7c6e856c2ca393004f1e8