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Freeze-fracture electron microscopy of lipid membranes on colloidal polyelectrolyte multilayer coated supports
- Source :
- Biomacromolecules 4 (2003): 808–814. doi:10.1021/bm034013r, info:cnr-pdr/source/autori:Moya, S.; Moya, S.; Richter, W.; Leporatti, S.; Bäumler, H.; Donath, E./titolo:Freeze-fracture electron microscopy of lipid membranes on colloidal polyelectrolyte multilayer coated supports/doi:10.1021%2Fbm034013r/rivista:Biomacromolecules/anno:2003/pagina_da:808/pagina_a:814/intervallo_pagine:808–814/volume:4
- Publication Year :
- 2003
- Publisher :
- American Chemical Society, Washington, DC , Stati Uniti d'America, 2003.
-
Abstract
- Lipid membranes were assembled on polyelectrolyte (PE)-coated colloidal particles. The assembly was studied by means of confocal microscopy, flow cytometry, scanning force microscopy, and freeze-fracture electron microscopy. A homogeneous lipid coverage was established within the limits of optical resolution. Flow cytometry showed that the lipid coverage was uniform. Freeze-fracture electron microscopy revealed that the lipid was adsorbed as a bilayer, which closely followed the surface profile of the polyelectrolyte support. Additional adsorption of polyelectrolyte layers on top of the lipid bilayer introduced inhomogeneities as evident from jumps in the fracture plane. Characteristic lipid multilayers have not been seen with freeze-fracture electron microscopy.
- Subjects :
- Materials science
Polymers and Plastics
Polymers
Bioengineering
Model lipid bilayer
law.invention
Biomaterials
Confocal microscopy
law
Polymer chemistry
Materials Chemistry
Freeze Fracturing
Colloids
Lipid bilayer
polyelectrolyte multilayer
electron microscopy
Bilayer
Resolution (electron density)
Freeze-fracture
Membranes, Artificial
Lipids
Polyelectrolyte
Microscopy, Electron
Membrane
Chemical engineering
lipids (amino acids, peptides, and proteins)
lipid membranes
Electron microscope
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules 4 (2003): 808–814. doi:10.1021/bm034013r, info:cnr-pdr/source/autori:Moya, S.; Moya, S.; Richter, W.; Leporatti, S.; Bäumler, H.; Donath, E./titolo:Freeze-fracture electron microscopy of lipid membranes on colloidal polyelectrolyte multilayer coated supports/doi:10.1021%2Fbm034013r/rivista:Biomacromolecules/anno:2003/pagina_da:808/pagina_a:814/intervallo_pagine:808–814/volume:4
- Accession number :
- edsair.doi.dedup.....e4c243c2d50bf11bed97d569f37c645b