Back to Search
Start Over
Lack of galectin-3 increases Jagged1/Notch activation in bone marrow-derived dendritic cells and promotes dysregulation of T helper cell polarization
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Galectin-3, an endogenous glycan-binding protein, is abundantly expressed at sites of inflammation and immune cell activation. Although this lectin has been implicated in the control of T helper (Th) polarization, the mechanisms underlying this effect are not well understood. Here, we investigated the role of endogenous galectin-3 during the course of experimental Leishmania major infection using galectin-3-deficient (Lgals3-/-) mice in a BALB/c background and the involvement of Notch signaling pathway in this process. Lgals3-/- mice displayed an augmented, although mixed Th1/Th2 responses compared with wild-type (WT) mice. Concomitantly, lymph node and footpad lesion cells from infected Lgals3-/- mice showed enhanced levels of Notch signaling components (Notch-1, Jagged1, Jagged2 and Notch target gene Hes-1). Bone marrow-derived dendritic cells (BMDCs) from uninfected Lgals3-/- mice also displayed increased expression of the Notch ligands Delta-like-4 and Jagged1 and pro-inflammatory cytokines. In addition, activation of Notch signaling in BMDCs upon stimulation with Jagged1 was more pronounced in Lgals3-/- BMDCs compared to WT BMDCs; this condition resulted in increased production of IL-6 by Lgals3-/- BMDCs. Finally, addition of exogenous galectin-3 to Lgals3-/- BMDCs partially reverted the increased sensitivity to Jagged1 stimulation. Our results suggest that endogenous galectin-3 regulates Notch signaling activation in BMDCs and influences polarization of T helper responses, thus increasing susceptibility to L. major infection. Fil: Fermino, Marise L.. Universidade de Sao Paulo; Brasil Fil: Dergan Dylon, Leonardo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Cecílio, Nerry T.. Universidade de Sao Paulo; Brasil Fil: Santos, Sofia N.. Comissão Nacional de Energia Nuclear. Centro de Radiofarmácia; Brasil Fil: Toscano, Marta Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Dias Baruffi, Marcelo. Universidade de Sao Paulo; Brasil Fil: Roque Barreira, Maria C.. Universidade de Sao Paulo; Brasil Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Bernardes, Emerson S.. Comissão Nacional de Energia Nuclear. Centro de Radiofarmácia; Brasil
- Subjects :
- 0301 basic medicine
CIENCIAS MÉDICAS Y DE LA SALUD
Galectin 3
Blotting, Western
Immunology
Notch signaling pathway
Leishmaniasis, Cutaneous
Bone Marrow Cells
Endogeny
Inflammation
Stimulation
Biology
Real-Time Polymerase Chain Reaction
LEISHMANIA MAJOR
Mice
03 medical and health sciences
medicine
Animals
DIFERENCIAÇÃO CELULAR
Leishmania major
T HELPER RESPONSE
Molecular Biology
Mice, Knockout
GALECTIN-3
Mice, Inbred BALB C
Receptors, Notch
NOTCH SIGNALING
Cell Differentiation
Patología
Dendritic Cells
T-Lymphocytes, Helper-Inducer
T helper cell
Bioquímica y Biología Molecular
Flow Cytometry
biology.organism_classification
Cell biology
Disease Models, Animal
Medicina Básica
030104 developmental biology
medicine.anatomical_structure
Galectin-3
Bone marrow
medicine.symptom
Jagged-1 Protein
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....e4a6c57fc3d0bfb93926412660fc99ec