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26-Locus Y-STR typing in a Bhutanese population sample

Authors :
Emma J. Parkin
Thirsa Kraayenbrink
George L. van Driem
Karma Tshering of GaselĂ´
Peter de Knijff
Mark A. Jobling
Source :
Forensic Science International. 161:1-7
Publication Year :
2006
Publisher :
Elsevier BV, 2006.

Abstract

26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).

Details

ISSN :
03790738
Volume :
161
Database :
OpenAIRE
Journal :
Forensic Science International
Accession number :
edsair.doi.dedup.....e48b724ef841a19e67ce27f12bfe9557
Full Text :
https://doi.org/10.1016/j.forsciint.2005.10.008