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26-Locus Y-STR typing in a Bhutanese population sample
- Source :
- Forensic Science International. 161:1-7
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- 26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).
- Subjects :
- Male
Genetics
education.field_of_study
Chromosomes, Human, Y
Amelogenin
STR multiplex system
Population
Haplotype
Locus (genetics)
Biology
DNA Fingerprinting
Polymerase Chain Reaction
Pathology and Forensic Medicine
Genetics, Population
Dental Enamel Proteins
Gene Frequency
DNA profiling
Tandem Repeat Sequences
Multiplex polymerase chain reaction
Humans
Y-STR
Multiplex
Bhutan
education
Law
Subjects
Details
- ISSN :
- 03790738
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- Forensic Science International
- Accession number :
- edsair.doi.dedup.....e48b724ef841a19e67ce27f12bfe9557
- Full Text :
- https://doi.org/10.1016/j.forsciint.2005.10.008