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Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept

Authors :
Emilio Gomez-Gonzalez
Olga Muñoz
Juan Carlos Gomez-Martin
Jesus Aceituno-Castro
Beatriz Fernandez-Muñoz
Jose Manuel Navas-Garcia
Alejandro Barriga-Rivera
Isabel Fernandez-Lizaranzu
Francisco Javier Munoz-Gonzalez
Ruben Parrilla-Giraldez
Desiree Requena-Lancharro
Pedro Gil-Gamboa
José Luis Ramos
Cristina Rosell-Valle
Carmen Gomez-Gonzalez
Maria Martin-Lopez
Maria Isabel Relimpio-Lopez
Manuel A. Perales-Esteve
Antonio Puppo-Moreno
Francisco Jose Garcia-Cozar
Lucia Olvera-Collantes
Silvia de los Santos-Trigo
Emilia Gomez
Rosario Sanchez-Pernaute
Javier Padillo-Ruiz
Javier Marquez-Rivas
Source :
Journal of Quantitative Spectroscopy and Radiative Transfer. 302:108567
Publication Year :
2023
Publisher :
Elsevier BV, 2023.

Abstract

Objective: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Methods: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were preparations in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-{\mu}L droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry of 87 samples at 405 nm and 514 nm. A polarimetric camera was used for simultaneous imaging of several samples under 380-420 nm illumination at angles similar to those of maximal DLP. A per-pixel image analysis included quantification and combination of polarization feature descriptors in other 475 samples. Results: The angles (from sample surface) of maximal DLP were 3 degrees for 405 nm and 6 degrees for 514 nm. Similar viral particles that differ only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned from polarimetric image analysis at 10-degree and 15-degree configurations. Conclusion: Polarimetric imaging in the visible spectrum has the potential for non-contact, reagent-free detection of viruses in multiple dry fluid residues simultaneously. Further analysis including real SARS-CoV-2 in human samples -- particularly, fresh saliva -- are required. Significance: Polarimetric imaging under visible light could contribute to fast, cost-effective screening of SARS-CoV-2 and other pathogens.

Details

ISSN :
00224073
Volume :
302
Database :
OpenAIRE
Journal :
Journal of Quantitative Spectroscopy and Radiative Transfer
Accession number :
edsair.doi.dedup.....e47f66dbfa947e26f988ec792acfaf5f