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Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits
- Source :
- Plant Cell
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Engineering improved Rubisco for the enhancement of photosynthesis is challenged by the alternate locations of the chloroplast rbcL gene and nuclear RbcS genes. Here we develop an RNAi-RbcS tobacco (Nicotiana tabacum) master-line, tobRrΔS, for producing homogenous plant Rubisco by rbcL-rbcS operon chloroplast transformation. Four genotypes encoding alternative rbcS genes and adjoining 5′-intergenic sequences revealed that Rubisco production was highest (50% of the wild type) in the lines incorporating a rbcS gene whose codon use and 5′ untranslated-region matched rbcL. Additional tobacco genotypes produced here incorporated differing potato (Solanum tuberosum) rbcL-rbcS operons that either encoded one of three mesophyll small subunits (pS1, pS2, and pS3) or the potato trichome pS(T)-subunit. The pS3-subunit caused impairment of potato Rubisco production by ∼15% relative to the lines producing pS1, pS2, or pS(T). However, the βA-βB loop Asn-55-His and Lys-57-Ser substitutions in the pS3-subunit improved carboxylation rates by 13% and carboxylation efficiency (CE) by 17%, relative to potato Rubisco incorporating pS1 or pS2-subunits. Tobacco photosynthesis and growth were most impaired in lines producing potato Rubisco incorporating the pS(T)-subunit, which reduced CE and CO(2)/O(2) specificity 40% and 15%, respectively. Returning the rbcS gene to the plant plastome provides an effective bioengineering chassis for introduction and evaluation of novel homogeneous Rubisco complexes in a whole plant context.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Operon
Ribulose-Bisphosphate Carboxylase
Nicotiana tabacum
Arabidopsis
Context (language use)
Plant Science
Biology
Rhodospirillum rubrum
Photosynthesis
01 natural sciences
In Brief
03 medical and health sciences
Bacterial Proteins
Gene Expression Regulation, Plant
Tobacco
Peptide Chain Initiation, Translational
Research Articles
Solanum tuberosum
fungi
RuBisCO
food and beverages
Cell Biology
Plants, Genetically Modified
biology.organism_classification
Plant Leaves
Chloroplast
Protein Subunits
Transformation (genetics)
030104 developmental biology
Chloroplast DNA
Biochemistry
biology.protein
RNA Interference
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1532298X and 10404651
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- The Plant Cell
- Accession number :
- edsair.doi.dedup.....e46b3fe2ede20cb4478943103df9e067