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An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides
- Source :
- Sensors (Basel, Switzerland), Sensors, Volume 19, Issue 23, Sensors, Vol 19, Iss 23, p 5111 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- A toxicity electrochemical DNA biosensor has been constructed for the detection of carcinogens using 24 base guanine DNA rich single stranded DNA, and methylene blue (MB) as the electroactive indicator. This amine terminated ssDNA was immobilized onto silica nanospheres and deposited on gold nanoparticle modified carbon-paste screen printed electrodes (SPEs). The modified SPE was initially exposed to a carcinogen, followed by immersion in methylene blue for an optimized duration. The biosensor response was measured using differential pulse voltammetry. The performance of the biosensor was identified on several anti-cancer compounds. The toxicity DNA biosensor demonstrated a linear response range to the cadmium chloride from 0.0005 ppm to 0.01 ppm (R2 = 0.928) with a limit of detection at 0.0004 ppm. The biosensor also exhibited its versatility to screen the carcinogenicity of potential anti-cancer compounds.
- Subjects :
- Guanine
Carcinogenesis
Oligonucleotides
DNA, Single-Stranded
Metal Nanoparticles
02 engineering and technology
macromolecular substances
Biosensing Techniques
Cadmium chloride
lcsh:Chemical technology
01 natural sciences
Biochemistry
Article
Analytical Chemistry
chemistry.chemical_compound
Electrochemistry
Humans
lcsh:TP1-1185
Electrical and Electronic Engineering
Instrumentation
Electrodes
Detection limit
010405 organic chemistry
Oligonucleotide
technology, industry, and agriculture
toxicity
electrochemical
DNA
Electrochemical Techniques
021001 nanoscience & nanotechnology
Silicon Dioxide
Atomic and Molecular Physics, and Optics
Carbon
0104 chemical sciences
carcinogen
Methylene Blue
chemistry
Carcinogens
Differential pulse voltammetry
Gold
0210 nano-technology
Biosensor
Methylene blue
DNA biosensor
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14248220
- Volume :
- 19
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Sensors (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....e459ca18883fa0ec7a43ea8c526fce34