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Ultra-High Resolution 3D Imaging of Whole Cells
- Source :
- ResearcherID, Cell
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Summary Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.<br />Graphical Abstract<br />Highlights • Whole-cell 4Pi single-molecule switching nanoscopy allows 10- to 20-nm 3D resolution • Refined hardware and new data analysis allow imaging of cells as thick as ∼10 μm • Using structure-averaging, the 3D shape of a bacteriophage can be resolved • Wide applicability across diverse research fields is demonstrated<br />A new super-resolution microscope opens a window deep into cells to image organelles and subcellular structures spanning large volumes.
- Subjects :
- 0301 basic medicine
Resource
Male
Cytological Techniques
Golgi Apparatus
Nanotechnology
02 engineering and technology
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Mice
Spermatocytes
Microscopy
Animals
Bacteriophages
Atomic de Broglie microscope
Extramural
Biochemistry, Genetics and Molecular Biology(all)
Synaptonemal Complex
Resolution (electron density)
Depth direction
021001 nanoscience & nanotechnology
Ultra high resolution
Single Molecule Imaging
030104 developmental biology
Microscopy, Fluorescence
COP-Coated Vesicles
0210 nano-technology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- ResearcherID, Cell
- Accession number :
- edsair.doi.dedup.....e455f5d26f4221db4d9c334863cdf428