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Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing
- Source :
- FEBS Letters. 588:1024-1030
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.
- Subjects :
- Mrna expression
Biophysics
Biochemistry
Umbilical vein
Mediator
Endothelial cell
Downregulation and upregulation
Cell Movement
Stress, Physiological
Structural Biology
Human Umbilical Vein Endothelial Cells
Genetics
Humans
Actin-binding protein
Molecular Biology
Cells, Cultured
Wound Healing
Shear stress
biology
Chemistry
Microfilament Proteins
Cell Biology
Biomechanical Phenomena
Cell biology
Endothelial stem cell
Immunology
biology.protein
Synaptopodin
Endothelium, Vascular
Transcriptome
Wound healing
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 588
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....e44e5033f02fa70e510a37c4df8b0668