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Identification of a 60S Preribosomal Particle that Is Closely Linked to Nuclear Export
- Source :
- Molecular Cell. 8(3):517-529
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- A nuclear GTPase, Nug1p, was identified in a genetic screen for components linked to 60S ribosomal subunit export. Nug1p cosedimented with nuclear 60S preribosomes and was required for subunit export to the cytoplasm. Tagged Nug1p coprecipitated with proteins of the 60S subunit, late precursors to the 25S and 5.8S rRNAs, and at least 21 nonribosomal proteins. These included a homologous nuclear GTPase, Nug2p, the Noc2p/Noc3p heterodimer, Rix1p, and Rlp7p, each of which was implicated in 60S subunit export. Other known ribosome synthesis factors and proteins of previously unknown function, including the 559 kDa protein Ylr106p, also copurified. Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export.
- Subjects :
- Ribosomal Proteins
Saccharomyces cerevisiae Proteins
Protein subunit
Recombinant Fusion Proteins
Molecular Sequence Data
Active Transport, Cell Nucleus
GTPase
Saccharomyces cerevisiae
Biology
Ribosome
GTP Phosphohydrolases
Fungal Proteins
Transformation, Genetic
Genes, Reporter
Centrifugation, Density Gradient
Animals
Humans
Amino Acid Sequence
Nuclear pore
Nuclear export signal
Molecular Biology
Cell Nucleus
Eukaryotic Large Ribosomal Subunit
Nuclear cap-binding protein complex
Temperature
Nuclear Proteins
Cell Biology
Ribosomal RNA
Blotting, Northern
Molecular biology
Cell biology
Ribosomes
Sequence Alignment
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....e44b481c2ca9fb8fece553190d275244
- Full Text :
- https://doi.org/10.1016/s1097-2765(01)00342-2