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A rapid procedure for the determination of phenyl-alanine and tyrosine from blood filter paper specimens
- Source :
- Clinica Chimica Acta. 31:447-452
- Publication Year :
- 1971
- Publisher :
- Elsevier BV, 1971.
-
Abstract
- A method is described for rapid analysis of phenylalanine and tyrosine using eluates of whole blood that has been impregnated into filter paper. This method employs semi-automated ion-exchange column chromatography (amino acid analyzer) and will yield reasonably accurate and consistent quantitative values for phenylalanine and tyrosine concentrations within two hours. The technique has significant value in rapidly confirming the presence of increased phenylalanine with or without a concomitant increase in tyrosine concentration in autoclaved or unautoclaved blood filter paper specimens. Thus phenylketonuria can be differentiated from hyperphenylalaninemia due to tyrosinemia within hours after the initial identification of an elevated phenylalanine concentration by a screening method such as the Guthrie test. In addition, further evaluations of phenylalanine and tyrosine concentrations can be facilitated in infants on whom only blood filter paper specimens are obtainable.
- Subjects :
- Paper
Hot Temperature
Phenylalanine
Clinical Biochemistry
Biochemistry
Tyrosinemia
Column chromatography
Hyperphenylalaninemia
Phenylketonurias
medicine
Animals
Humans
Horses
Tyrosine
Amino Acid Metabolism, Inborn Errors
Whole blood
Alanine
Autoanalysis
Chromatography
Filter paper
Chemistry
Biochemistry (medical)
General Medicine
Chromatography, Ion Exchange
medicine.disease
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Clinica Chimica Acta
- Accession number :
- edsair.doi.dedup.....e444075cea4bf7682cc0601997c56c7c