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In vitroproduction of cyclic AMP and steroids from an ovarian Sertoli-Leydig cell tumor: Notes on clinical management
- Source :
- Acta Obstetricia et Gynecologica Scandinavica. 74:310-317
- Publication Year :
- 1995
- Publisher :
- Wiley, 1995.
-
Abstract
- A 27 year old nulliparous woman with a history of chronic anovulation and signs of virilization with a markedly elevated serum level of testosterone, underwent a laparotomy with peroperative bilateral ovarian vein catheterization and bilateral bisection of both ovaries. A solid, 1.5 cm, well delimited tumor located centrally in the right ovary, was excised. Testosterone levels in ovarian venous blood from the tumor bearing side, were 88.4 nmol/l and from the contralateral ovary 3.9 nmol/l. Histopathological examination showed a Sertoli-Leydig cell tumor which was radically extirpated. Postoperatively, the serum levels of androgen normalized, the woman had regular cycles, became pregnant and delivered a normal female baby. Pieces of tumor tissue were incubated for 2 h, with and without addition of gonadotropins and adrenocorticotropic hormone (ACTH). Human chorionic gonadotropin (CG), follicle stimulating hormone (FSH) and adrenocorticotropic hormone (ACTH) caused significant increases in cyclic monophosphate (cAMP) production in tumor tissue in vitro, as compared to controls. Furthermore, ACTH also significantly stimulated 17 beta-estradiol production. In tumor cells cultured for 48 h, FSH slightly, but not significantly, increased the production of progesterone. In the cell culture, [3H]-thymidine incorporation into deoxyribonucleic acid (DNA) was stimulated by IGF1 alpha but not by hCG and FSH. It is concluded that Sertoli-Leydig cell tumors may be sensitive to gonadotropins and ACTH and that their small size, solid shape and intra-ovarian localization can cause diagnostic difficulties.
- Subjects :
- Adult
endocrine system
medicine.medical_specialty
medicine.drug_class
Ovary
Adrenocorticotropic hormone
Chorionic Gonadotropin
Human chorionic gonadotropin
Anovulation
Sertoli-Leydig Cell Tumor
Follicle-stimulating hormone
Adrenocorticotropic Hormone
Pregnancy
Culture Techniques
Internal medicine
Cyclic AMP
Tumor Cells, Cultured
medicine
Humans
Testosterone
Postoperative Period
Insulin-Like Growth Factor I
Progesterone
Ovarian Neoplasms
Estradiol
Leydig cell
business.industry
Androstenedione
Infant, Newborn
Obstetrics and Gynecology
General Medicine
medicine.disease
Endocrinology
medicine.anatomical_structure
Female
Follicle Stimulating Hormone
Gonadotropin
business
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 16000412 and 00016349
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Acta Obstetricia et Gynecologica Scandinavica
- Accession number :
- edsair.doi.dedup.....e40c239004887f42dbe3d138b5cfa8d0