Analysis of indoleamine 2,3-dioxygenase 1 (IDO1) expression of cultured cord blood adherent mononuclear cells as an indicator of atopic risk

* Correspondence: 8yz7@queensu.ca Department of Biomedical and Molecular Sciences/Medicine, Queen’s University, Kingston, ON, K7L 3N6, Canada Figure 1 IDO1 gene expression fold changes relative to plain media control. IDO1 expression levels were normalized to HPRT1 expression. The error bars represent the standard error of the mean. Numbers per stimulation group are as indicated beneath the graph. Cultures of atopic and non-atopic AMNCs were plated at 7.5x10 cells per condition. Following 5.5 hours incubation with either plain media, 1 μg/ml IFN-g, or 1 μg/ml IFN-g and 10 ng/ml CSE, cells were lysed for RNA extraction. RNA was reverse transcribed and cDNA levels were analyzed. Zhu et al. Allergy, Asthma & Clinical Immunology 2014, 10(Suppl 1):A72 http://www.aacijournal.com/content/10/S1/A72 ALLERGY, ASTHMA & CLINICAL IMMUNOLOGY